摘要
目的探讨微小RNA-215参与胃癌中的表达及其临床意义。方法应用实时荧光定量聚合酶链式反应法检测微小RNA-215在胃癌细胞和组织中的表达,分析其表达与胃癌细胞分化程度、临床病理特征的关系。结果微小RNA-215的表达在胃癌细胞中高于正常胃黏膜细胞株,其表达量与胃癌细胞的分化程度之间无明显相关性;在胃癌组织中微小RNA-215与胃癌的分化程度、临床病期分类分期及淋巴结转移等密切相关。结论微小RNA-215可能发挥癌基因的作用参与胃癌的发生、发展进程。
Objective To investigate the role of miRNA-215 involved in the development of gastric cancer.Methods Polymerase chain reaction were used to detect the expression of miRNA-215in gastric cancer tissue and human gastric cancer cell,and its association with clinicopathologic features and cell differentiation were analyzed.Results The expression of miRNA-215 was higher in gastric cancer cells than in gastric mucosa GES-1 cells.There were no correlation found between the microRNAs expression and differentiated grades of gastric cancer.miRNA-215 was closely related to differentiated grades,stages and lymph node metastasis of gastric cancer in the gastric cancer tissues.Conclusion miRNA-215 may play an important role in the development process of gastric cancer.
出处
《兰州大学学报（医学版）》
CAS
2013年第4期13-16,20,共5页
Journal of Lanzhou University（Medical Sciences）
基金
甘肃省卫生行业科研计划(GSWST09-03)
"西部之光"人才培养计划[(2012)18号]
