远缘物种DNA导入水稻保持系的种质创新及SSR分析

SSR Analysis of the Variants from Rice Maintainer Lines Transferred Exogenous DNA

将小粒野生稻 (Oryzaminuta)、高粱的DNA分别导入水稻保持系V2 0B和IR5 80 2 5B ,在第 1代 (D1 )获得变异 ,从前者变异株的后代中选育出了新的不育系及其保持系株系野威A和野威B ,而高粱DNA导入IR5 80 2 5B获得的变异系从D2 开始在形态上已不出现分离。SSR分析表明 ,变异系野威B、香粱 5均与受体存在遗传多态性 ,并含有供体特异的分子标记带型。首次从分子水平上证明 ,通过导入外源DNA获得的变异株确实存在无分离的现象。说明导入远缘物种DNA是创造水稻新种质的有效途径。

The new CMS line Yewei A and its maintainer line Yewei B were developed from the variant of V20B, a very popular rice maintainer line, into which the genomic DNA of O.minuta was transformed. Three variants were also selected from another maintainer line, IR58025B, after the introduction of the genomic DNA of sorghum. From the D_2 generation of one of the three variant plants on, no morphological segregation has been found among its different filial plants.This variant is denoted as ″Xiangliang 5″. The results of SSR analysis showed that there existed much polymorphism of repetitive DNA sequence between the variants and the receptors, with special DNA segments from distant relatives (donors) integrated into the genome of rice. The paper has first proven at the molecular level that some variants from transformation of exogenous DNA can be stablized in the second generation. It is also concluded that introduction of the genomic DNA from distant relatives is an effective way to create new germplasms in rice.