水稻温敏核不育突变体0A15-1的育性表现及遗传学研究

Sterility Investigation and Genetical Analysis of a Thermosensitive Genic Male Sterile Mutant 0A15-1 in Rice

水稻细胞质型雄性不育系IR69700A的幼穗经离体培养,获得一个体细胞克隆突变体0A15-1。经短日照和低温处理表明,0A15-1具有在高温下不育和低温下转为可育的特性,是一例温敏不育突变体。花粉染色显示0A15-1属于典败型不育。通过与明恢63、优B和广陆矮等多个父本的杂交,其F2和BC1群体的育性分离比都揭示0A15-1的不育性状受一对隐性核基因控制,并且为孢子体型雄性不育。该新种质可以用于对相关温敏核不育基因进行分子标记及用于两系法生产杂交水稻。

A somaclonal mutant 0A15-1 obtained from in vitro culture by young panicles of IR69700A showed typically abortive type of male sterility as proved by pollen staining with 1% iodide-potassium iodide solution. As shown by short day and low temperature treatments, the mutant had a character of thermosensitive genic male sterility which exhibited fertility conversation, i.e. sterile under high temperature and fertile under low temperature, regardless of being under long day or short day. Analysis of fertility segregation pattern from F1, F2 and BC1 indicated that the sterility trait of 0A15-1 was controlled by a single recessive gene and belonged to sporophytic male sterile type. This mutant is an useful material both for gene tagging and production of hybrid rice by means of photo(thermo)-sensitive male sterile lines.