高空气球搭载实验对普通鸡冠花抗氧化作用的诱变效应

The Effect of High Altitude Treatment on Antioxidation Activity of Water Extract from Celosia argentea L. (Yellow Flower)

利用高空气球搭载黄色普通鸡冠花(CelosiaargenteaL.)(yellowflower)种子,进行空间诱变处理。飞行高度为40.112km,飞行时间近4h,回收后播种栽培,采收子一代(SP1)的花序和叶片。采用比色法研究搭载组和对照组花序、叶片水提液对超氧阴离子自由基(O2-.)和羟自由基(·OH)的清除作用,并测定分析了样品的VC,黄酮醇的含量和SOD活力。结果表明,搭载组和对照组花序和叶片水提液均能降低·OH引发的还原型细胞色素C[Cyt·C(Ⅱ)]氧化作用和O2-.对羟胺的氧化作用,而搭载组样品水提取液清除活性氧的能力均比对照组显著提高,这与其自由基清除剂含量和活力的增高有关。这说明高空环境诱变处理对鸡冠花中抗氧化活性物质的合成和抗氧化功能产生了显著效应。

The seeds of Celosia argentea L.(yellow flower) were carried by a high altitude balloon(CHAB) at an altitude of 40.112 km above sea level for about 4 hours. After the balloon returned on the ground, the treated seeds were stored for 8 months at 4℃ and then sowed. The inflorescences and leaves of the first generation were used for water extraction. The effects of water extracts from inflorescences and leaves of treated and control plants on scavenging of superoxide radical (O2-. ) and hydroxyl radical (·OH) were studied by colorimetric method, and the contents of Vitamin C, flavonol and the activity of SOD of all samples were determined. The results showed that the water extracts could reduce ·OH induced oxidation of reductive Cyt·c (Ⅱ) and inhibit O2-. induced oxidation of hydroxylamine. The effects of water extracts from treated plants on scavenging active oxygen were more signifi-cant than those from the control, indicating that high altitude condition treatment could increase antioxidation activity in cockscombs.