水稻外源DNA导入系的创建及主要性状分析

Construction of Exogenous DNA Introgression Lines and Analysis of its Main Traits in Rice

通过花粉管通道法将稗草总体DNA和玉米总体DNA导入水稻受体R122中,获得的D1代变异频率分别高达1.487%和3.81%。对稳定的3个玉米DNA导入系和4个稗草DNA导入系研究表明,与受体R122相比,导入系在株型、株高、生育期、分蘖力、着粒密度、粒形、稃尖颜色、稻瘟病抗性、稻米品质、恢复力和耐储藏性等生物学性状方面发生了广泛的变异。同时讨论了外源DNA导入水稻的变异频率和在水稻育种中的应用前景。

The direct introduction of Maize (Zea Mays L.) DNA and barnyard grass (Echinochloa crusgalli) DNA into rice recipient R122 were carried out through pollen-tube-pathway method and the frequency of variation in D1 were 1.487% and 3.81%, respectively. Four stable maize DNA introgression lines and 4 stable barnyard grass DNA introgression lines were constructed and then investigated. The results suggested that, the introgression lines showed extensive changes, compared with the recipient R122, in biological traits, such as plant type, plant height, growth duration, tillering ability, spikelet setting density, grain shape, apiculus color, blast resistance, rice quality, restoring ability and storability characters. The frequency of variation and utilization of exogenous DNA introduction in rice breeding were also discussed.