NRDRiso酶cDNA的序列测定及生物信息学分析

Sequencing and Bioinformatic Analysis of NRDRiso cDNA

通过鉴定分析人肝组织中辅酶II依赖性视黄醇脱氢酶不同剪接体全长cDNA核苷酸序列与氨基酸序列的结构特征 ,为今后进一步研究体内维甲酸的代谢情况奠定基础。根据人、小鼠NRDR编码区的一致性序列 ,设计一对引物 ,应用RT PCR方法从人肝组织中得到一条 377bp的新的cDNA片段。采用RACE法得到了NRDR新亚型cDNA ,并以生物信息学软件分析其生物学特征。测序得知该cDNA长为 1 0 0 3bp ,以NADP dependentretinoldehydroge nase reductaseshortisoform(NRDRiso)登录GenBank。其读码框为 5 2 5bp ,拟编码 1

This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H) dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full length cDNA is 1,003bp in length and was submitted to GenBank as NADP dependent retinol dehydrogenase /reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.