摘要
为研究放射性核素经不同注射方法局部辐照后对瘤体治疗作用,建立了C 6胶质痛细胞皮下移植瘤大鼠模型, 分别采用直形注射针线状退行注射和弧形套针单点分段旋转扇形注射法,经皮向肿瘤内注射131Ⅰ-碘化油(0.1×37 MBq/cm3), 观察给药后一定时间段大鼠体重、肿瘤大小的变化和动物存活时间,同时以穿刺针和小剪刀取材按常规方法制备病理切片和超薄切片,观察两种给药方法肿瘤组织的形态学变化。结果表明,直形针给药组大鼠在注射后2周多数表现为体重下降,肿瘤增大,最后都死于全身耗竭,而弧形针组大鼠体重相对稳定,肿瘤组织表现为均匀坏死,缓慢崩溃,动物存活期明显比前者长;直形针给药组肿瘤组织早期表现为大片的坏死,晚期表现为存活区域的增加,坏死区域与存活区域界限分明,两者间多有移行带,电镜下可见到形态典型的低分化C6细胞;弧形针分段扇形注药者早期、晚期组织均表现为大片坏死,肿瘤间质内可见到淋巴细胞浸润和纤维结缔组织增生;各时间段的检测标本中都可见到一定数量的凋亡细胞,但比例远远小于坏死的肿痛细胞。结果提示,弧形针分段扇形注射是较为理想的给药方式;“坏死”是治疗剂量131Ⅰ-碘化油内放疗对痛体的主要效应。
To investigate the therapeutic effect of 131I-iodinated oil internal radiation on glioma, 131I-iodinated oil was injected sector-shapely into tumor model of C6 glioma in rat with arc-needle, matc hed with routine straight-needle injection. the therapeutic effect induced by 131I-iodinated oil intraleision radiation in the carcinoma models we rerecorded through survival time, body weight, the size of local carcinoma and detected by histopathological examination. The Results showed that the rats in arc-needle injection of 131I-iodinated oil in C6 glioma at subcutis could increase rat's survival time, the body weight kept less desent, the lumps necrosed wholely. Pathological and ultrastructural detection revealed that the cell necrosis and collapse, sever nuclear damage was observed in the death cells. The early characteristics of necrosis such as margination of heterochromatin was also found in some tumor cells. Besides, well differentiated tumor cells, deg enerative tumor cells and some lymphocytes were seen. Conclusion arc-needle inj ection of 131I-iodinated oil step-by step and sector-shapely into tumor was a better method, necrosis was the major effect of 131I-iodi nated oil internal radiation in carcinoma at the level of treated dosage.
出处
《上海实验动物科学》
2004年第2期88-90,127,共4页
Shanghai Laboratory Animal Science
