六道木属六道木组种间关系的AFLP分析和黄花六道木中国分布新记录的认定

Species Relationships in Abelia sect.Abelia (Caprifoliaceae) in East Asia and Verification of Distribution of A.serrata in China:Evidence from AFLP Analysis

用荧光标记AFLP技术对东亚产六道木属六道木组作了系统关系分析。我们采用 5对AFLP引物 ,对六道木属 9个物种 ,锦带花属和猬实属各 1个物种共 16份样品进行分析 ,共获得 988个位点。不同引物所获得的结果具有很高的相容性 ,Mantel相关系数为 0 72 5～0 919,说明AFLP技术适用于六道木属的种间关系分析。用邻接法 (neighbor joining)对 16个样品 988个位点的分析生成的系统树获得很高的自展分析 (bootstrap)支持率。树系图表明 ,猬实属与六道木属管花六道木组有密切的关系。在六道啪组内 ,温州六道木与糯米条的关系密切 ,单花六道木与大花六道木差异很小 ,黄花六道木与日本产的近缘种组成一单系分支。本文认定 ,根据形态特征鉴定浙江永嘉的标本为黄花六道木 (AbeliaserrataSieb .etZucc .)是正确的 ,属中国分布新记录。文章最后补充描述了中国产的黄花六道木。黄花六道木在中国的发现进一步证明中国植物区系与日本植物区系的密切关系。

In this paper AFLP technique was used to elucidate systematic relationships among the species of Abelia in East Asia.Seven species of Abelia sect.Abelia,2 species of Abelia sect.Zabelia,1 species of Kolkwitzia,and 1 species of Weigela were sampled.Five AFLP primer combinations produced 988 informative sites.High correlations between data sets of different primer combinations indicated that AFLP markers are suitable for revealing species relationships in Abelia.The neighbor-joining tree received high bootstrap support and showed a close alliance between Kolkwitzia amabilis and the species in Abelia section Zabelia.In section Abelia three clads were readily recognizable:(1)A.chinensis and A.spathulata;(2)A.grandiflora and A.uniflora;and(3)A.serrata and A.tetrasepala.Identification of specimens collected in eastern China to be A.serrata based on morphology was supported by AFLP analysis.Descriptions were emended for recognition of this species in China.The discovery of A.serrata in China shows again a very close biogeographic relationship between eastern China and Japan.