人疱疹病毒6型与淋巴瘤关系的实验研究

Relationship between human herpesvirus 6 and lymphoma

目的 研究人疱疹病毒 6型 (HHV 6 )感染与淋巴瘤的关系。 方法 用间接免疫荧光试验及聚合酶链反应 ,分别检测淋巴瘤患者和对照组 (其它血液病 )患者血清中抗HHV 6IgG及外周血单个核细胞中的HHV 6DNA序列。用免疫组织化学染色法检测淋巴瘤患者淋巴结组织标本中HHV 6抗原。 结果 淋巴瘤患者抗HHV 6IgG的阳性率为 95 5 % ,几何平均滴度为 1∶12 3,明显高于对照组 (P <0 0 5 )。淋巴瘤患者标本中HHV 6DNA的检出率明显高于对照组 (P <0 0 5 )。免疫组织化学染色法检测的 5例淋巴瘤患者的淋巴结组织标本中 ,4例HHV 6抗原阳性。 结论 HHV

Objective To study the role of human herpesvirus 6 (HHV 6) in pathogenesis of lymphoma. Methods The serum anti HHV 6 antibody titers from lymphoma patients and control subjects were detected by indirect immunofluorescence assay. HHV 6 DNA in peripheral blood mononuclear cells (PBMCs) from lymphoma patients and control subjects was amplified by PCR. An immunohistochemical staining using rabbit anti HHV 6 antibody was used to detect HHV 6 antigen in lymph nodes from lymphoma patients. Results Statistical analyses of the data revealed that detected rate (95.5%) and geometric mean titer (1∶123) of anti HHV 6 antibody in 22 lymphoma patients were higher than those (73.3% and 1:58) in 15 control subjects. There was statistically significant difference between the patients and control group for HHV 6 DNA in PBMCs. HHV 6 antigens were detected in 4 out of 5 lymph nodes from lymphoma patients. Conclusion These results demonstrate the frequent presence of HHV 6 in lymphoma, suggesting that HHV 6 infection may have a relation with pathogenesis of human lymphoma.