摘要
目的 在酵母细胞中表达IL-18。方法 用Sac I 将pPIC9K-IL18线性化后,电转化毕氏酵母GS115,筛选后得到His+Mut+转化子,在BMGY和BMMY培养基中进行诱导表达,表达产物进行SDS-PAGE和Western blot 分析。结果 SDS-PAGE和Western blot印迹杂交结果证实表达蛋白的相对分子量为21 000,与预期的分子量一致。结论 在毕氏酵母中成功的表达了IL-18。
Objective To express IL-18 in Pichia.pastoris .Methods The constructed plasmid pPIC9K-IL18 aslinarized with Sac I and transformed into Pichia.pastoris GS115. His+Mut+ transformants was screened outand expressed in BMGY and BMMY media. The espressed products were analyzed by SDS-PAGE andWestern blot .Results SDS-PAGE and Western blot showed a relative molecular weight of expressed proteinof about 21 000.Conclusion Human Il-18 protein was successfully expressed in Pichia .pastoris.[
出处
《中国医学生物技术应用》
2004年第1期32-35,共4页
The Chinese Academic Medical Magazine of Organisms
关键词
白细胞介素
毕氏酵母
表达
Interleukin Pichia.pastoris
Expression
