摘要
Stem segments of Dioscorea zingiberensis (Huang jiang) were used as explants which were cultured on B5 or MS medium supplemented with different plant hormones. The result indicated that combination of BA2+ NAA0.2 gave optimum effectiveness for the induction of callus(over50%), the induction rate of adventitious bud is 30~50%. Suitable rooting medium is MS + IBA 0.1 0.5 or 1/2 MS without hormone. The plantlets grew successfully when transplanted into soil mixed with perlite and vermiculite after training treatment.
Stem segments of Dioscorea zingiberensis (Huang jiang) were used as explants which were cultured on B5 or MS medium supplemented with different plant hormones. The result indicated that combination of BA2+ NAA0.2 gave optimum effectiveness for the induction of callus(over50%), the induction rate of adventitious bud is 30~50%. Suitable rooting medium is MS + IBA 0.1 0.5 or 1/2 MS without hormone. The plantlets grew successfully when transplanted into soil mixed with perlite and vermiculite after training treatment.
出处
《中国医学生物技术应用》
2002年第1期41-43,共3页
The Chinese Academic Medical Magazine of Organisms