摘要
遗传标记(源于DNA序列差异的特征)可以通过很多不同的途径显示出来。随着生化与分子生物技术的发展完善,可以从分子水平上直接显示它的变化-分子标记。分子标记技术具有可靠性,高效性, 因而被广泛应用于多种研究领域。分子标记通常包括同工酶(或蛋白质)和DNA标记。本文综述生化和DNA标记(蛋白/酶,RFLP, RAPD, AFLP, SSR,SNP, SSLP等)技术的原理、发展及应用。
Genetic markers, derived from differences in the DNA sequence , can be observed in several different way.With the improvement of biochemistry and molecular biology techniques it can directly be detected at the molecularlevel. That is the molecular marker techniques. They have been widely used in many research fields due to their reliabil-ity and efficiency. The molecular marker, in general, include Isozymes(or protein)markers and DNA markers. Thispaper mainly interpret biochemistry markers and DNA markers, such as RFLP, RAPD, AFLP, SSR, SNP and SSLP etc,and briefly outline the basic principles of the techniques, their advantages/disadvantages and application.
出处
《中国医学生物技术应用》
2002年第2期33-40,共8页
The Chinese Academic Medical Magazine of Organisms
基金
山东省教育厅基金(J01S08)
莱阳农学院基金(000-3)
关键词
生物遗传多样性
分子标记
PCR
organism genetic diversity,molecular markers, Southern blotting,PCR
