摘要
目的探讨骨桥蛋白在体重不一致性双胎胎盘中的表达变化。方法用Western blotting及实时荧光定量PCR检测双胎盘胎盘中的骨桥蛋白的表达。结果体重不一致DCDA双胎的小胎与大胎胎盘比较,Western blotting结果显示,体重不一致DCDA双胎的小胎与大胎胎盘比较,胎盘中蛋白表达明显减少(0.31±0.09VS 0.62±0.08,t=-4.182,P=0.001),体重一致DCDA双胎胎盘间OPN蛋白表达无明显差异(0.62±0.16VS 0.68±0.13,t=-1.687,P=0.724);实时荧光定量PCR结果显示,体重不一致DCDA双胎的小胎与大胎胎盘比较,胎盘中OPN mRNA表达明显减少(0.61±0.28VS1.01±0.12,t=3.087,P=0.014),体重一致DCDA双胎胎盘间OPN mRNA表达无明显差异(0.98±0.18VS 1.02±0.11,t=1.884,P=0.464)。Western blotting显示体重不一致MCDA的小胎与大胎比较,OPN蛋白表达显著下降,(0.24±0.06VS 0.73±0.18,t=5.687,P<0.001),体重一致MCDA双胎胎盘间OPN蛋白表达无明显差异(0.48±0.12VS 0.42±0.15,t=-1.687,P=0.586);实时荧光定量PCR结果显示,体重不一致MCDA的小胎与大胎胎盘比较,OPN mRNA表达显著下降,(0.46±0.16VS 1.01±0.09,t=5.128,P<0.001),生长一致MCDA双胎胎盘间OPN mRNA表达无明显差异(0.89±0.15VS 0.95±0.22,t=0.697,P=0.564)。结论体重不一致性双胎胎盘中的骨桥蛋白表达有显著生差异,小胎胎盘骨桥蛋白的表达量明显减少。
Objective To analyze the expression of OPN in placenta of discordant twins,and explore the relationship between the OPN expression and pathophysiology of discordant twins.Method Real-time quantitat ive PCR reaction,Western blotting,immunohistochemistry,were used to detect the expression level of OPN mRMA and protein in placenta.Results As to the discordant DCDA twin,placenta of small fetus compared with that of large fetus,Western blotting results showed that OPN protein expression was significantly decreased in placenta of small fetus(0.31±0.09VS 0.62±0.08,P<0.05);OPN protein expression was not significantly different in the placenta between the concordant DCDA twins(0.62±0.16VS 0.68±0.13,P>0.05);real-time quantitative PCR results showed that OPN mRNA expression was significantly decreased in placenta of small fetus for discordant DCDA twins(0.61±0.28VS 1.01±0.12,P<0.05),OPN mRNA expression was not significantly different in the placenta between the concordant DCDA twins(0.98±0.18VS 1.02±0.11,P>0.05).As to the discordant MCDA twin,placenta of small fetus compared with that of large fetus,Western blotting results showed that OPN protein expression was significantly decreased in placenta of small fetus(0.24±0.06VS 0.73±0.18,P<0.05);OPN protein expression was not significantly different in the placenta between the concordant DCDA twins(0.48±0.12VS 0.42±0.15,P>0.05);real-time quantitative PCR results showed that OPN mRNA expres-sion was significantly decreased in placenta of small fetus for discordant DCDA twins(0.46±0.16VS 1.01±0.09,P<0.05),OPN mRNA expression was not significantly different in the placenta between the concordant DCDA twins(0.89±0.15VS 0.95±0.22,P>0.05).Conclusions The expression of OPN mRNA and correlated with twin placental territory,suggesting that OPN involved in placental devel- opment,low OPN expression may be associated with discordant twins.
出处
《中国产前诊断杂志（电子版）》
2013年第3期14-20,共7页
Chinese Journal of Prenatal Diagnosis(Electronic Version)
