新型可降解输尿管支架管材料的生物相容性研究

Biocompatibility Testing of A New Biodegradable Ureteral Stent

目的通过初步的生物相容性实验,评估纤维基可降解输尿管支架管的生物相容性。方法我们选用了体外细胞毒性实验和大鼠肌肉埋植实验评估其生物相容性。其中体外细胞毒性试验被分为4组:阴性对照组(不放材料)、阳性对照组(天然乳胶材料的浸提液)和两组实验组(两种不同制作条件下支架管材料的浸提液:150℃组和210℃组)。选用第4代尿源性细胞种植于96孔板中,种植细胞24、72、120小时后,用CCK-8试剂盒测定细胞增值活力。肌肉埋植实验是将各支架管材料和阳性对照试件埋入SD大鼠双侧臀肌中,各试件将于埋植术后1、4、12周后取出。通过组织学分析观察肌肉组织对试件的反应,并评价各试件的组织相容性。结果两种输尿管支架材料生物相容性良好,对尿源性细胞无毒性。支架管材料为非细菌炎性反应,12周后材料周围组织基本恢复正常。结论纤维基可降解输尿管支架管具有良好的细胞和组织生物相容性。

Objective To evaluate the biocompatibility properties of the novel braided biodegradable ureteral stents. Methods The ureteral stent materials were examined by tests for in vitro cytotoxicity and muscle implantation tests. The test for cytotoxicity was divided into 4 groups: negative control (no materials), positive control (extract medium from natural latex), two different experimental groups (extract medium from different stents:150℃ group and 210℃ group).Urine derived cells of generation 4 were implanted in 96-hole-plank. Cell viability was evaluated by using cell counting kit-8 after an incubation period of 24h、72h、120h,respectively. Pellets made of ureteral stents and natural latex were implanted in the gluteus of SD rats. Tissue blocks surrounding the biomaterials were taken out after 1, 4,12 weeks, respectively. Histological analysis was done to investigate the tissue reaction and evaluate their tissue biocompatibility. Results Two ureteral materials were biocompatible and exerted no toxic effect on urine derived cells.Non-infectious reactions due to materials implanted were observed. The tissue aroud ureteral stents was basically normal after 12 weeks. Conclusions Our biodegradable materials has good cytocompatibility and tissue biocompatibility.