CPPS患者前列腺液细菌16SrRNA基因及白细胞数与临床疗效的相关性研究

The Correlation of 16 SrRNA Gene and WBC in EPS of Men with CPPS and Clinical Curative Effect

目的探讨应用聚合酶链反应(PCR)检测慢性非细菌性前列腺炎(CPPS)患者前列腺液中细菌16SrRNA基因,并常规检查患者前列腺液中白细胞的数目,与CPPS患者的临床治疗效果做相关性分析。方法以细菌16SrRNA基因为靶序列,设计引物及寡核苷酸探针,采用PCR法检测标准菌株及135例CPPS患者前列腺液中细菌16SrRNA基因,结合患者前列腺液中白细胞的数目进行对比分析。结果 135例CPPS患者中细菌16SrRNA基因检测结果阳性为78例,阳性率为57.78%。细菌16SrRNA基因阳性组患者经抗生素治疗的有效率84.6%,阴性组有效率52.6%;细菌16SrRNA基因阳性组患者疗效明显优于阴性组。按白细胞数分组疗效比较,不同组间疗效没有差异。结论大部分CPPS患者的前列腺液中可以检测到细菌16SrRNA基因,提示细菌感染在CPPS的发病中有重要作用。但是,其前列腺液中白细胞数目对CPPS患者判断病情、估计预后无统计学意义。

Objective To study the correlation of 16S rRNA gene and WBC detected by PCR in Expressed prostatic secretion(EPS)of man with CPPS and clinical curative effect.Methods The 16S rRNA and WBC in EPS from 135 patients with CPPS.The curative effect was evaluated by NIH-CPSI before the treatment or three month later.The SPSS Chi-Square test,and Logistic regression analysis program were used for statistic analysis. Significance was considered as P<0.05.Results Of the 135 CPPS patients,positive 16SrRNA signals were detected in 78 samples,and negative in 57 samples.The positive 16SrRNA signals were detected in 3 samples of the health control,negative in 18 samples of the group.Significant difference was seen in positive rate between CPPS group and health control group.Significant difference of curative effect was seen in improvement between positive 16 SrRNA signal and negative group.No significant difference was found in CPPS groups with different WBC in'EPS..Conclusions In our findings,bacteria 16 SrRNA can be detected in the most CPPS patients'EPS which indicates that bacteria play an important role in chronic prostatitis.We also hope that the results of this study will encourage further research on the role of bacteria in all categories of prostatitis syndrome.Moreover, the number of leukocytes in ESP doesnt show statistical significance in estimating prognosis