摘要
Objective To detect the expression of Nanog in glioma cell line U87 and the relationship with BTSCs. Methods BTSCs were isolated from glioma cell line U87 andcultured in simplified serum-free neural stem cell medium by nanosphere suspension culture method spheres,and purified continuously through the monoclonal formation experiment. The immunofluorescence staining of cells was employed
出处
《外科研究与新技术》
2011年第3期208-208,共1页
Surgical Research and New Technique