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Expression and significance of multi-drug resistance-associated protein 3 in different tumor cell lines

Expression and significance of multi-drug resistance-associated protein 3 in different tumor cell lines
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摘要 Objective To investigate the expression and meaning of MRP3 in different tumor cells. MethodsThe monoclonal antibody against MRP3 was used to identify the expression of MRP3 by flow cytometer in seven tumor cells and human embryo kidney cell lines 293T.And RT-PCR was used to detect the mRNA of MRP3 in eight cell lines. ResultsThe mRNA of MRP3 was expressed in three pancreatic carcinoma cell lines.MRP3 protein was observed in BxPC-3 and AsPC-1 cells. ConclusionMRP3 may express in different tumor in tissue-specific manner.BxPC-3 and AsPC-1 may serve as cellular models for in vitro studies on multidrug resistance of pancreatic carcinoma. Objective To investigate the expression and meaning of MRP3 in different tumor cells. MethodsThe monoclonal antibody against MRP3 was used to identify the expression of MRP3 by flow cytometer in seven tumor cells and human embryo kidney cell lines 293T.And RT-PCR was used to detect the mRNA of MRP3 in eight cell lines. ResultsThe mRNA of MRP3 was expressed in three pancreatic carcinoma cell lines.MRP3 protein was observed in BxPC-3 and AsPC-1 cells. ConclusionMRP3 may express in different tumor in tissue-specific manner.BxPC-3 and AsPC-1 may serve as cellular models for in vitro studies on multidrug resistance of pancreatic carcinoma.
作者 张辉 高玮 王从俊 尤天庚
机构地区 General Surgery of Dongfang Hospital
出处 《外科研究与新技术》 2010年第1期59-62,共4页 Surgical Research and New Technique
关键词 MULTIDRUG resistance-associated PROTEIN TUMOR CELL expression multidrug resistance-associated protein tumor cell expression
分类号 R73-3 [医药卫生—肿瘤]
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参考文献10

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外科研究与新技术
2010年 第1期

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