Bone tumor

209370 Gene profiling of MAPK pathway in human osteosarcoma/Li Guodong(李国东,Dept Orthop,10th Peop Hosp Affil Tongji Univ,Shanghai 200433)…∥Chin J Oncol.-2009,31(5).-340～345Objective To explore the functional effects of MAPK pathway in the pathogenesis of human osteosarcoma.Methods Gene microarray(Human Genome U133A,Affymetrix)was used to screen the differential expression of genes involved in MAPK pathway between osteosarcoma cell lines and 3 osteoblastic cell lines.KEGG metabolic pathway analysis was performed among significantly increased or decreased genes using the MATLAB software.Immunohistochemical technique was used to detect the expressions of ERK1/2,JNK and p38 proteins among 48 osteosarcoma and benign 24 osteoblastic tumor samples.Results Using an entrance limit of ≥2.0,18 differentially expressed MAPK pathway-related genes were selected(10 up-regulated,8 down-regulated)to mapped to the MAPK pathway of KEGG which are all important node genes.The positive rates of ERK1/2,JNK and p38 proteins were 83.3%(40/48),72.9%(35/48)and 85.4%(41/48)in osteosarcomas,and 12.5%(3/24),8.3%(2/24)and 16.7%(4/24)in the control group,respectively.The positive rates and expression intensities were statistically different between the 2 groups(P【0.01).Conclusion MAPK pathway plays an important role in the pathogenesis of osteosarcoma.ERK,JNK and p38 form and intercoodinating network and regulate the cell proliferation,differentiation,apoptosis,invasion and migration in osteosarcoma.15 refs,2 figs,2 tabs.