金属蛋白酶-2及其组织抑制因子在原发性胆囊癌的表达及意义(英文)

EXPRESSION AND ITS SIGNIFICANCE OF MMP-2 AND TIMP-2 PROTEINS IN GALLBLADDER CARCINOMAS

目的 探讨MMP - 2、TIMP - 2蛋白在原发性胆囊癌的表达及意义。方法 应用免疫组化SABC法结合图像分析技术 ,定量检测 4 5例胆囊癌和 15例胆囊息肉样病变的MMP - 2和TIMP - 2蛋白表达。结果 胆囊癌MMP - 2表达量显著高于胆囊息肉样病变 (P =0 .0 0 2 ) ;MMP - 2表达与胆囊癌组织学类型、分化程度、浸润深度、淋巴结转移及肝脏浸润等均无相关性 ,但不同分期的MMP - 2表达量差异显著 ;胆囊癌TIMP - 2表达虽与胆囊息肉样病变无差异 (P =0 .2 95 ) ,但不同Nevin分期间、浸润深度不同、有无局部淋巴结转移及肝脏浸润的胆囊癌 ,TIMP - 2表达量有显著差异。结论 TIMP - 2表达能较准确地反映胆囊癌的生物学特点 ,有可能作为一个新的标志 ,在胆囊癌的早期诊断、浸润转移的判断和患者预后的估计有重要的意义。

Objective To study the relationships between expression of MMP-2 and TIMP-2 protein and clinical-pathological parameters in gallbladder carcinomas.Methods Carcinomas ( n =45) and polypoid lesions of the gallbladder ( n =15) were studied.Expression of MMP-2 and TIMP-2 protein was examined by immunohistochemical avidin-biotin-complex method and the image analysis.Clinical-pathological dates in patients with carcinomas of the gallbladder such as histological type,grade of differentiation,level of infiltration,liver invasion and lymph node involvement,etc were recorded.Results The average level (1.123±0.108 vs 1.0301±0.054,P =0.002) of MMP-2 expression was significantly higher in carcinoma of the gallbladder than in polypoid lesions of the gallbladder.The significant difference was found between the expression of MMP-2 in early stage and advanced tumors.But there are no correlations between MMP-2 in early stage and advanced tumors.But there are no correlations between MMP-2 protein expression and histological type,differentiation degree,infiltration level,lymph node involvement or liver invasion.Though correlation was not observed between TIMP-2 expression and histological type or differentiation degree,the significant relationship was found between TIMP-2 expression and different Nevin stage,infiltration level,local lymph node involvement or liver invasion in patients with carcinoma of the gallbladder ( P <0.05).Conclusion Expression of TIMP-2 protein could reflect more accurately biological character of gallbladder carcinomas when compared with MMP-2.If united examination,MMP-2 and TIMP-2 could help to differentiate malignant lesions from benign ones of the gallbladder and TIMP-2 might be a significant clinical directors in the judgment of invasion or metastasis and the estimating of prognosis in patients with carcinoma of the gallbladder.