Studies on transplantation of bone marrow stromal cells transferred by angiogenin gene in ischemic myocardium

Objective To evaluate the protective effect of bone marrow stromal cells (BMSCs) transferred by AD·ANG ex vivo on ischemic myocardium. Methods ELISA method was used to assay the expression and secretion of angiogenin (ANG) after Ad·ANG transfection of BMSCs ex vivo. Then BMSCs with Ad · ANG were transplanted into ischemic myocardium of isogenic Lewis rats. 4 weeks later, the parameters of hear function, such as EF and EDLV, were examined by echocardiography. Surival and differentiation of transplanted BMSCs and angiogenesis were appraised by histology and transmission electron electron micrography. Results ANG was found in both lysate and culture medium after transfection of BMSCs. A maximum expression of ANG was observed at 4-7 days after transfection and could still be assayed 15 days later. 4 weeks later after transplantation in the BMSCs with Ad· NAG, heart function improved better than the single BMSCs group (P【0.05), Ad·ANG group (P【0.01)and blank control group (P【0.01). Angiogenesis in

Objective To evaluate the protective effect of bone marrow stromal cells (BMSCs) transferred by AD·ANG ex vivo on ischemic myocardium. Methods ELISA method was used to assay the expression and secretion of angiogenin (ANG) after Ad·ANG transfection of BMSCs ex vivo. Then BMSCs with Ad · ANG were transplanted into ischemic myocardium of isogenic Lewis rats. 4 weeks later, the parameters of hear function, such as EF and EDLV, were examined by echocardiography. Surival and differentiation of transplanted BMSCs and angiogenesis were appraised by histology and transmission electron electron micrography. Results ANG was found in both lysate and culture medium after transfection of BMSCs. A maximum expression of ANG was observed at 4-7 days after transfection and could still be assayed 15 days later. 4 weeks later after transplantation in the BMSCs with Ad· NAG, heart function improved better than the single BMSCs group (P<0.05), Ad·ANG group (P<0.01) and blank control group (P<0.01). Angiogenesis in test group was more obvious than three control groups( P<0. 01). Conclusion Satisfactory expression of ANG can obtained Ad · ANG was transferred into BMSCs ex vivo. After transplantation of BMSCs with Ad ·ANG into ischemic myocardium, improvement of heart function and angiogenesis are more obvious than sigle BMSCs transplantation and Ad·ANG injection. 5 refs,5 figs.