摘要
Objective To investigate and evaluate a novel fluorotyping procedure for HLA-DRB1 locus. Methods On the basis of low resolutation SSP technique, the fluorogenic probe was used to establish the fluorotyping procedure of HLA-DRB1 which was carried out for 46 samples. Results The fluorotyping has been sucessful for all the 46 samples and was completely coincident to the SSP results. As compared to the serological typing of DR locus,the coincidence rate was 66. 3% (61/92) whereas the coincidence rate of both the 2 specific genes was 43. 5% (20/46). Conclusion Fluorotyping is accurate, sensitve, reproducible, depends less on manual manipulation and eliminates the problems related to contamination. 10 refs.
Objective To investigate and evaluate a novel fluorotyping procedure for HLA-DRB1 locus. Methods On the basis of low resolutation SSP technique, the fluorogenic probe was used to establish the fluorotyping procedure of HLA-DRB1 which was carried out for 46 samples. Results The fluorotyping has been sucessful for all the 46 samples and was completely coincident to the SSP results. As compared to the serological typing of DR locus,the coincidence rate was 66. 3% (61/92) whereas the coincidence rate of both the 2 specific genes was 43. 5% (20/46). Conclusion Fluorotyping is accurate, sensitve, reproducible, depends less on manual manipulation and eliminates the problems related to contamination. 10 refs.
出处
《外科研究与新技术》
2003年第2期113-113,共1页
Surgical Research and New Technique
