Correlation between tumor necrosis factor a gene promoter polymorphisms and acute rejection following renal transplantation

Objective To study the significance of cytokine tumor necrosis factor α ( TNF -α) gene promoter 308 position polymorphisms in predicting acute graft rejection following renal transplantation. Methods In 35 preoperative recipients, TNF-α produced by peripheral blood cells was measured by enzyme-linked immmunosorbent assay, and their TNF- α gene promoter 308 position polymorphisms were determined by FOR restriction fragment length polymorphisms(PCR-RFLP). The assocication between TNF-α gene promoter polymorphisms and production of them was studied. Furthermore, the correlation between their polymorphisms and acute rejection in the first 3 months after renal transplantation was discussed. Results The recipients with A/A or A/G genotype in TNF-α promoter 308 position secreted more cytokine (624.96 ± 177.78) pg/ml and (544.32 ± 13.242)pg/ml than those with G/G(233.16 ± 25.37)pg/ml,P【0.01. When HLA-DR was mismatched, the recipients with high production of TNF - α genotype showed higher incidence

Objective To study the significance of cytokine tumor necrosis factor α ( TNF -α) gene promoter 308 position polymorphisms in predicting acute graft rejection following renal transplantation. Methods In 35 preoperative recipients, TNF-α produced by peripheral blood cells was measured by enzyme-linked immmunosorbent assay, and their TNF- α gene promoter 308 position polymorphisms were determined by FOR restriction fragment length polymorphisms(PCR-RFLP). The assocication between TNF-α gene promoter polymorphisms and production of them was studied. Furthermore, the correlation between their polymorphisms and acute rejection in the first 3 months after renal transplantation was discussed. Results The recipients with A/A or A/G genotype in TNF-α promoter 308 position secreted more cytokine (624.96 ± 177.78) pg/ml and (544.32 ± 13.242)pg/ml than those with G/G(233.16 ± 25.37)pg/ml,P<0.01. When HLA-DR was mismatched, the recipients with high production of TNF - α genotype showed higher incidence rate of acute rejection (50%) than those with low production of genotype (8 % ). Conclusion TNF-α gene promoter polymorphisms are significantly related with cytokine production of stimulated peripheral blood cells and are correlation with acute rejection in the first 3 months following renal transplantation. Determination of TNF-α gene promoter 308 genotype of renal transplantation recipients before operation has certain clinical significance in predicting risk of acute rejection. 8 refs.