miR-21在巨噬细胞对MRSA感染过程中免疫应答的调控

The Control of miR-21 in the Immune Response of Macrophages on MRSA Infections

目的建立耐甲氧西林金黄色葡萄球菌(MRSA)的体外感染模型,研究miR-21的生物学功能及其在巨噬细胞中对MRSA感染过程中的免疫应答调控机制,以期为临床提供更多关于MRSA感染的相关免疫应答信息,为临床治疗提供参考数据和新的治疗思路。方法利用前期冻存的高效表达成熟miR-21的重组腺病毒颗粒pAd/pri-miR-21感染小鼠巨噬细胞RAW264.7。利用MRSA感染已经侵染腺病毒颗粒pAd/pri-miR-21的细胞,建立感染模型,Real-Time PCR检测TLR4、NF-κB和IL-6 mRNA水平的表达。结果 miR-21可下调TLR4基因的表达,降低TLR/4Myd88/NF-κB下游分子NF-κB、IL-6的活性。结论证实miR-21对TLR/4Myd88/NF-κB信号通路具有负向调控作用,在MRSA感染的巨噬细胞的免疫调控中发挥着重要的作用。

Objective To establish a vitro infection model with Methicillin-resistant Staphylococcus aureus(MRSA),to study the biological function of miR-21 and its regulatory mechanism in the process of immune response to MRSA infection of macrophages,in order to provide more information about MRSA infections associated immune response for clinical reference data and new ideas in clinical practice.Methods The pre-cryopreservation of energy efficient expression of mature miR-21recombinant adenovirus particles pAd/pri-miR-21 infect RAW264.7.The attack poison of MRSA has infected the cells of the recombinant adenovirus,established the MRSA in vitro infection model,the Real-Time PCR detection of TLR4,NF-κB and the expression of IL-6 mRNA level.Results MiR-21 can down-regulate the expression of TLR4 gene,reduce the activity of downstream molecule NF-κB,IL-6 in TLR/4Myd88/NF-κB.Conclusion The results confirms that miR-21 has a negative regulatory role on the in immune regulation the TLR/4Myd88/NF-κB signaling pathway and plays an important role in controlling of MRSA infection of macrophages.