摘要
BACKGROUND; In recent years, extracorporeal liver per- fusion (ECLP) has been regarded as a treatment of acute liver failure ( ALF ); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a sta- ble and effective system of ECLP. METHODS; Livers were harvested from health pigs, ac- cording to the different styles of perfusion and oxygena- tion, which were randomly divided into 3 groups. The liv- ers in group A (n =4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n =4) to dual ( portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C ( n = 4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemody- namic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. RESULTS: The perfusion time of the liver in group A is significantly shorter than in groups B and C (P<0.05). At 1 , 3 , 6 hours after perfusion, the data of bile production and hemodynamic parameters of livers in group A were sta- tistically different from those of livers in groups B and C (P<0.05). At 1, 3, 6 hours after perfusion, the data of group B were not statistically different from those of livers in group C (P>0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of liv- ers in group C (P<0.01). CONCLUSION: The system of ECLP, which is performed by dual ( portal vein and hepatic artery) vessel perfusionand oxygenating blood separately, is more stable and effec- tive to keep the function of extracorporeal liver.
BACKGROUND; In recent years, extracorporeal liver per- fusion (ECLP) has been regarded as a treatment of acute liver failure ( ALF ); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a sta- ble and effective system of ECLP. METHODS; Livers were harvested from health pigs, ac- cording to the different styles of perfusion and oxygena- tion, which were randomly divided into 3 groups. The liv- ers in group A (n =4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n =4) to dual ( portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C ( n = 4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemody- namic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. RESULTS: The perfusion time of the liver in group A is significantly shorter than in groups B and C (P<0.05). At 1 , 3 , 6 hours after perfusion, the data of bile production and hemodynamic parameters of livers in group A were sta- tistically different from those of livers in groups B and C (P<0.05). At 1, 3, 6 hours after perfusion, the data of group B were not statistically different from those of livers in group C (P>0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of liv- ers in group C (P<0.01). CONCLUSION: The system of ECLP, which is performed by dual ( portal vein and hepatic artery) vessel perfusionand oxygenating blood separately, is more stable and effec- tive to keep the function of extracorporeal liver.
作者
Jin Gong, Xi-Mo Wang, Gang Long, Zhong-Tao Guo, Tao Jiang and Shi Chen Wuhan, ChinaInstitute of Organ Transplantation, Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030, China Department of Surgery, Tianjin People’s Hospital, Tianjin 300120, China
基金
This study was supported by grants from the National High Technology Re-search and Development Program Foundation of China(863 Program) ( No.2001AA216071 )and from the Science & Technology Development Founda-tion of Tianjin Health Bureau ( Vo. 2002KY35).
