注射用丹参酮ⅡA磺酸钠的制备及其对神经元的药理活性研究

Preparation of sodium tanshinone ⅡA sulfonate for injection and its pharmacological activity on neurons

目的制备注射用丹参酮ⅡA磺酸钠,建立神经元缺氧模型,考察丹参酮ⅡA磺酸钠对缺氧造成的神经元损伤的保护作用。方法以质量浓度为80mg·mL-1的甘露醇作为支撑剂,冷冻干燥法制备注射用丹参酮ⅡA磺酸钠。体外培养大鼠皮层神经元,建立神经元缺氧模型,给药后24h采用MTT法测定神经元细胞的活性。结果制备的冻干粉针复溶性良好,浓度为0.1、1.0、10μmol·L-1的丹参酮ⅡA磺酸钠可显著提高缺氧神经元细胞的活性。结论注射用丹参酮ⅡA磺酸钠对缺氧造成的神经元损伤具有保护作用,可用于治疗因脑缺氧引起的疾病。

Objective To prepare sodium tanshinone IIA sulfonate for injection and to study the effects of sodium tanshinone IIA sulfonate on rat cortical neuron damage induced by ischemia. Methods 80 mg·mL-1mannitol was selected as excipient. Sodium tanshinone IIA sulfonate for injection was prepared by freeze-drying. Rat cortical neuron was cultivated in vitro, and ischemia model of neuron was established. Cell viability was detected by MTT method at 24 h after administration. Results The clarity was good after sodium tanshinone IIA sulfonate redissolved in water. Sodium tanshinone IIA sulfonate (0.1, 1.0, 10 μmol·L-1) could increase cell activity of neuron. Conclusion Sodium tanshinone IIA sulfonate for injection can protect neuron cell against damage. It may be used to treatment brain ischemia disease in clinical.