摘要
目的制备注射用丹参酮ⅡA磺酸钠,建立神经元缺氧模型,考察丹参酮ⅡA磺酸钠对缺氧造成的神经元损伤的保护作用。方法以质量浓度为80mg·mL-1的甘露醇作为支撑剂,冷冻干燥法制备注射用丹参酮ⅡA磺酸钠。体外培养大鼠皮层神经元,建立神经元缺氧模型,给药后24h采用MTT法测定神经元细胞的活性。结果制备的冻干粉针复溶性良好,浓度为0.1、1.0、10μmol·L-1的丹参酮ⅡA磺酸钠可显著提高缺氧神经元细胞的活性。结论注射用丹参酮ⅡA磺酸钠对缺氧造成的神经元损伤具有保护作用,可用于治疗因脑缺氧引起的疾病。
Objective To prepare sodium tanshinone IIA sulfonate for injection and to study the effects of sodium tanshinone IIA sulfonate on rat cortical neuron damage induced by ischemia. Methods 80 mg·mL-1mannitol was selected as excipient. Sodium tanshinone IIA sulfonate for injection was prepared by freeze-drying. Rat cortical neuron was cultivated in vitro, and ischemia model of neuron was established. Cell viability was detected by MTT method at 24 h after administration. Results The clarity was good after sodium tanshinone IIA sulfonate redissolved in water. Sodium tanshinone IIA sulfonate (0.1, 1.0, 10 μmol·L-1) could increase cell activity of neuron. Conclusion Sodium tanshinone IIA sulfonate for injection can protect neuron cell against damage. It may be used to treatment brain ischemia disease in clinical.
出处
《中国药剂学杂志(网络版)》
2008年第1期1-5,共5页
Chinese Journal of Pharmaceutics:Online Edition
关键词
药剂学
冷冻干燥
MTT法
丹参酮ⅡA磺酸钠
pharmaceutics
lyophillization
MTT method
sodium tanshinone IIA sulfonate