圣尔康胶囊对链霉素肾病病活模型疗效的研究

The Experimental Study ofCaps.Shenerkang on Adriamycin Induced Nephrotic Syndrome in Rats

目的:探讨圣尔康对肾病的治疗作用。方法:将SD雄性大鼠90只随机分为6组:正常对照组、模型组、阳性药保肾康片组,圣尔康胶囊高剂量组、圣尔康胶囊中剂量组、圣尔康胶囊低剂量组。以链霉素(streptomycin,SM)诱导大鼠肾病模型。造模后24小时开始给药,疗程为三周。以一般状况、肾脏脏器系数、尿蛋白、血红蛋白、血肌酐(Cre-S)、尿素氮(BUN)及甘油三酯(Trig)等为观察指标,观察圣尔康胶囊对鼠肾病模型的治疗作用;取肾组织做光镜检查,观察肾脏组织结构的变化。结果:链霉素注射后,造模组大鼠尿蛋白、血红蛋白、肌酐、尿素氮及甘油三酯水平均较正常组显著增加,提示肾病模型制成。病理结果显示肾小管上皮细胞出现水肿及空泡样病变。给予圣尔康胶囊治疗3周后后,大鼠一般状况显著改善,Cre-S、BUN、Trig、大鼠尿液量、尿蛋白、微量游离血红蛋白及肾脏脏器系数水平同模型组相比均有明显好转(P<0.05)。病理组织学检查则显示,圣尔康胶囊可有效减轻肾小管水肿及空泡变的作用,使肾脏病变程度有所减轻。结论:圣尔康胶囊各治疗组对肾病大鼠均有一定的治疗作用。

Objective: To study theraputic effects of Caps.Shenerkang on streptomycin induced nephrotic syndrome in rats.Methods: Ninety SD rats were selected and randomly divided into six groups: the normal control group, the model group, baoshenkang group, Caps.Shenerkang group with low dosage, Caps.Shenerkang group with middle dosage and Caps.Shenerkang group with high dosage. The nephrotic syndrome model was established by intravenous injection of streptomycin, followed by baoshenkang or Caps.Shenerkang therapy. The normal group and the model group were given 0.9% sodium chloride. Three weeks after treatment,the normal conditions of rats,the renal index, urine protein quantity,urine volume, free hemoglobin,plasma creatinine,urea nitrogen,triglyceride,and pathological changes of renal tissues were observed. Result: Compared with the normal control group, urine protein quantity,free hemoglobin,plasma creatinine,urea nitrogen and triglyceride in model group were inereased. Pathological studies showed that the edema of renal tubular epithelial cell and vacuolar degeneration were taken placed. These results indicated that the nephrotic syndrome model was established. After three weeks treatment with Caps.Shenerkang, the normal conditions were significantly improved, and the renal index, urine protein quantity,urine volume, free hemoglobin,plasma creatinine,urea nitrogen and triglyceride level were all ameliorated comapared with the model group (P<0.05). The edema and vacuolar degeneration of nephrotic syndrome rats treated with Caps.Shenerkang were also improved.Conlusion: The results showed that Caps.Shenerkang exerted the theraputic effects on nephrotic syndrome