茶多酚对甲醛致人内皮细胞氧化损伤的保护作用

ProtectiveEffect of Tea Polyphenols on Oxidative Damaged Endothelial Cells Induced by Formaldehyde

目的探讨茶多酚对甲醛致人内皮细胞氧化损伤的保护作用。方法采用体外常规培养内皮细胞,取对数生长期的细胞进行实验。实验设对照组、甲醛损伤组和低、中、高剂量茶多酚保护组,其中茶多酚先与细胞预孵1 2h后加入0.1 mmoL/L甲醛,置CO2培养箱培养4h,检测细胞培养液中SOD活力及LDH、MDA、N0的含量,s P法检测NF-KB。结果茶多酚各保护组与甲醛损伤组比较SOD活力明显升高,LDH、MDA含量均降低(P<0.05);茶多酚各保护组内皮细胞N0释放量减少、NF—KB表达明显下降与甲醛损伤组比较均有显著性(P<0.05)。结论茶多酚可提高内皮细胞抗氧化酶的活性,减轻甲醛所致的氧化损伤;茶多酚通过抑制NF—KB的表达,减少N0的产生从而减轻细胞内脂质过氧化是其保护作用的重要机制。

Objective To investigate the protective effect of tea polyphenols on oxidative damaged endothelial cells induced by Formaldehyde.Methods Human umbilical vein endothelial cells(HUVEC-12)were cultured with DMEM in vitro and the cells in the exponential phase of growth to experiment were selected.They were divided into nomal cells group formaldehyde-induced injury group(0.1 mmol/L),low,medium and high doses of Tea Polyphenols protection group.The cells were pre-incubated with tea polyphenols,after 12h.adding 0.1 mmol/L formaldehyde and put in C02 Incubators foster home for 4h,then the SOD activity and LDH,MDA and NO contention of the cell culture medium were detected,method of SP was used to detect NF-KB.Results SOD activity of the tea Polyphenols protection groups were higher than the formaldehyde injury group actively;LDH,MDA levels were lower than formaldehyde-induced injury group,the differences were statistically significant(P<0.05);The NO levels of tea Polyphenols protection groups were lower,NF-KB's activity declined actively compared with the formaldehyde-induced injury group significantly.the differences were statistically significant(P<0,05). Conclusion Tea polyphenols can increase the activity of antioxidant enzymes of endothelial cells and reduce oxidative damage caused by fomaldehyde;The important mechanism of tea polyphenols' protective effection is to inhibit the expression of NF-KB and reduce NO generation which lightens the intracellular lipid peroxidation.