鼻敏片对变应性鼻炎大鼠模型NGF与SP的影响及其相互作用机制的研究

Experimental Study of Substance P and Nerve Growth Factor Intervenient Effects of BiMin Tab on Models Allergic Rhinitis In Rat

目的:探讨鼻敏片对实验性变应性鼻炎(allergic rhinitis,AR)影响及NGF、SP在变应性鼻炎中的作用,深入了解AR的发病机制。方法:将Wistar大鼠60只按完全随机分组,设正常对照组、阳性对照组、实验组1、实验组2、西药对照组。采用卵清蛋白制做大鼠变应性鼻炎模型,观察鼻部症状并按评分标准给予评分记录。取鼻粘膜涂片观察嗜酸性粒细胞含量;取血测嗜酸性粒细胞含量;取鼻粘膜进行免疫组化分析。结果:鼻敏片能降低大鼠P物质、NGF含量,能减轻变应性鼻炎大鼠鼻腔症状,P物质、SGF含量呈正相关。结论:P物质、NGF含量的异常变化是AR发病的重要因素和环节。以健脾温肾固表的鼻敏片通过影响NGF、SP含量水平,从而达到减轻AR鼻部症状,调节免疫功能的目的。

Objective: To investigate the therapeutic effects of BiMin Tab on experimental allergic rhinitis in rats and the intervenient effects of substance P and NGF.Methods: 60 white big rats were randomly divided into six groups: Normal group, model group, treating group 1, treating group 2 and control group. We replicate rat model of allergic rhinitis induced by perfusion of ovalbumin. After observing the syndrome of the rats, we use nasal mucosa to doing immunohistochemistry analysis.Result: BiMin Tab can relief the syndrome of the rats and decrease the number of the EOS cells in the rat’s nasal mucosa. ; the density of the substance P and NGF with the severity of the syndrome of the rat’s nasal mucosa. Conclusion: Substance P and NGF are important factors , and it play an important role in the development of allergic rhinitis . BiMin Tab is effective in the treatment of allergic rhinitic by regulating expression and release of substance P and NGF.