摘要
Objective:To investigate the potential antioxidant effects of Phyllanthus niruri(P.niruri.Euphorhiaceae)tea on healthy subjects.Methods:Five non-smoking,male healthy volunteers,20 to 31 years old.were enrolled.Kach subject was treated twice,following a randomized crossover fashion regarding the ingestion of P.niruri infusion(5 g/750 mL)(tea group)or 750 mL of water(control group).Fasting venous blood samples were collected prior to and at 1.2 and 4 h after infusion drinking.Samples were tested for plasmatic gallic acid and ascorbic acid levels,erythrocytic catalase and superoxide dismutase activities,and intracellular DCFH fluorescence in granulocytes,monocytes and lymphocytes.Results:Catalase and superoxide dismutase activities were not altered by tea ingestion.Plasma levels of gallic acid were significantly increased at1,2 and 4 h after P.niruri ingestion and plasma ascorbic acid at 1 h after P.niruri ingestion.Conclusions:Ingestion of P.niruri tea is associated with a slight increase in antioxidant markers in human blood(ascorbic acid and gallic acid),which may contribute to its pharmacological effects.
Objective:To investigate the potential antioxidant effects of Phyllanthus niruri(P.niruri.Euphorhiaceae)tea on healthy subjects.Methods:Five non-smoking,male healthy volunteers,20 to 31 years old.were enrolled.Kach subject was treated twice,following a randomized crossover fashion regarding the ingestion of P.niruri infusion(5 g/750 mL)(tea group)or 750 mL of water(control group).Fasting venous blood samples were collected prior to and at 1.2 and 4 h after infusion drinking.Samples were tested for plasmatic gallic acid and ascorbic acid levels,erythrocytic catalase and superoxide dismutase activities,and intracellular DCFH fluorescence in granulocytes,monocytes and lymphocytes.Results:Catalase and superoxide dismutase activities were not altered by tea ingestion.Plasma levels of gallic acid were significantly increased at1,2 and 4 h after P.niruri ingestion and plasma ascorbic acid at 1 h after P.niruri ingestion.Conclusions:Ingestion of P.niruri tea is associated with a slight increase in antioxidant markers in human blood(ascorbic acid and gallic acid),which may contribute to its pharmacological effects.
基金
finaneially supported by CNPq
