摘要
Objective:To analyze a phylogenetic tree for understanding the molecular systematic of trematode in Pamily Heterophyidae.which are highly distributed in Thailand.Methods:Based on thirteen sequences of mitochondrial cytochrome C oxidase subunit 1(mCO I) gene from six genera of heterophyid trernatodes,riz.Haplorchis,Stellantchasmus,Centrocestus,Metagonimus,Pygidopsis,and Haplnrchoides were aligned automatically using the Clustal × 2.0 program.A phylogenetic tree was constructed by maximum likeiughood(ML) and neighbor-joining(NJ)methods,with 1000 bootstrap using the 5.0 program.Results:The phylogenetic relationship from both methods was similar and separated into three groups consisting of Haplorchoides pumilio group,Haplorchoides taichui group and another heterophyid genera.Comclusions:The sequence data of mtCOl can he used to investigate the phylogenetic relationships of trernatodes at the genus level.Each clade of different genera of heterophyid trernatodes can be separated into sister groups that correlated with the morphological characteristic,kind of secondary intermediate host and geographic distribution.
Objective:To analyze a phylogenetic tree for understanding the molecular systematic of trematode in Pamily Heterophyidae.which are highly distributed in Thailand.Methods:Based on thirteen sequences of mitochondrial cytochrome C oxidase subunit 1(mCO I) gene from six genera of heterophyid trernatodes,riz.Haplorchis,Stellantchasmus,Centrocestus,Metagonimus,Pygidopsis,and Haplnrchoides were aligned automatically using the Clustal × 2.0 program.A phylogenetic tree was constructed by maximum likeiughood(ML) and neighbor-joining(NJ)methods,with 1000 bootstrap using the 5.0 program.Results:The phylogenetic relationship from both methods was similar and separated into three groups consisting of Haplorchoides pumilio group,Haplorchoides taichui group and another heterophyid genera.Comclusions:The sequence data of mtCOl can he used to investigate the phylogenetic relationships of trernatodes at the genus level.Each clade of different genera of heterophyid trernatodes can be separated into sister groups that correlated with the morphological characteristic,kind of secondary intermediate host and geographic distribution.
基金
supported by the Graduate School.Chiang Mai University
