摘要
Objective:To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria （L.siceraria）（Molina） fruit.Methods:The free radical scavenging activity of the L siceraria （Molina） fruit extract was assayed by usingα,α-diphenyl-β-picrylhydrazyl（DPPH）. 2,20-azinobis 3-ethyl henzothiazoline-6-sulfonate（ABTS）,FRAP,reducing power,chelating ability andβ-carotene bleaching assay.Results:The IC<sub>50</sub>values of DPPH and ABTS radicalscavenging activity was found to be 1.95 mg/mL and 19 mg/mL,respectively.In ferrous chelation assay,the percentage of inhibition was found to be 89.21%.The reducing power of ethanolic extract of L siceraria（Molina） fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL Theβ-carotene linoleate bleaching assay was 46.7%at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL Conclusions:The results indicate that L siceraria （Molina） fruit could be an important sources of natural radical scavengers.
Objective:To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria （L.siceraria）（Molina） fruit.Methods:The free radical scavenging activity of the L siceraria （Molina） fruit extract was assayed by usingα,α-diphenyl-β-picrylhydrazyl（DPPH）. 2,20-azinobis 3-ethyl henzothiazoline-6-sulfonate（ABTS）,FRAP,reducing power,chelating ability andβ-carotene bleaching assay.Results:The IC50values of DPPH and ABTS radicalscavenging activity was found to be 1.95 mg/mL and 19 mg/mL,respectively.In ferrous chelation assay,the percentage of inhibition was found to be 89.21%.The reducing power of ethanolic extract of L siceraria（Molina） fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL Theβ-carotene linoleate bleaching assay was 46.7%at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL Conclusions:The results indicate that L siceraria （Molina） fruit could be an important sources of natural radical scavengers.
基金
supported by University Grants Commission(UGC-File No. 2678/09(MRP/UGC-SERO),New Dclhi.India
