摘要
Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphylococcus aureus.16 specimens of coagulasenegative Staphylococci.2 specimens of Enterococci.3 specimens of Streptococcus,1 specimen of Micrococcus.3 specimens of Escherichia coli.4 specimens of Klebsiella pneumoniae,3 specimens of Pseudomonas aeruginosa,2 specimens of Enlerohacter cloacae.and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification(LAMP) assay.A total of118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.Results:Bv designing primers specific for Staphylococcus aureus,specimens containing different kinds of pathogens were earned out by LAMP assay,and our data showed LAMP technology lor the specific detection of Staphylococcus aureus in samples was successlully established.All clinical Specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP,and our data showed that compared wit blood culture method,the LAMP technology, showed significantly high detection rale(P 【0.01).Conclusions:As a quick and easy detection of Staphylococcus aureus,the LAMP technology was successfully established,laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis,and showed great promotion and application value.
Objective:To explore a rapid diagnostic method in neonatal sepsis and bacterial meningitis. Methods:The primers were designed and synthesized hased on 16S rRNA gene of Staphylococcus aureus.Four specimens of Staphylococcus aureus.16 specimens of coagulasenegative Staphylococci.2 specimens of Enterococci.3 specimens of Streptococcus,1 specimen of Micrococcus.3 specimens of Escherichia coli.4 specimens of Klebsiella pneumoniae,3 specimens of Pseudomonas aeruginosa,2 specimens of Enlerohacter cloacae.and 5 specimens of Acinetobacter were tested by loop-mediated isothermal amplification(LAMP) assay.A total of118 clinical specimens of sepsis and non-sepsis were collected and detected with both LAMP assay and blood culture.Results:Bv designing primers specific for Staphylococcus aureus,specimens containing different kinds of pathogens were earned out by LAMP assay,and our data showed LAMP technology lor the specific detection of Staphylococcus aureus in samples was successlully established.All clinical Specimens of sepsis and non-sepsis were tested by both blood cultures and LAMP,and our data showed that compared wit blood culture method,the LAMP technology, showed significantly high detection rale(P <0.01).Conclusions:As a quick and easy detection of Staphylococcus aureus,the LAMP technology was successfully established,laid the foundation for the diagnosis and treatment of children Staphylococcus aureus sepsis,and showed great promotion and application value.
