Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn,on streptozotocin induced diabetic rats

Objective:To evaluate wound healing potential of flavonoid fractions of Martynia annua (M.annua) Linn,leaves in diabetic rats on the basis of folkloric information and preliminary study.Methods:The flavonoid compound luteolin and apigenin were isolated from dried leaves of plant by column chromatography.The two concenlrations(0.2%and 0.5%w/w) of luteolin and flavonoid fraction were selected for topically applied as ointment on diabetic wound. The Povidone Iodine Ointment USP was used as a reference.On 18th days,protein content, hydroxyproline and antioxidants(SOD,CAT and GSH) level in granuloma tissues were determined. Results:The results showed that,percent wound contraction were observed significantly(P【0.01) greater in MAF fraction and 0.5%w/w of luteolin treatment groups.Presence of matured collagen fibres and fibroblasts with better angiogenesis were observed in histopathological studies. Conclusions:In conclusion,our findings suggest that flavonoid fraction(MAF) and luteolin(0.5% w/w) may have potential benefit in enhancing wound healing in diabetic condition,possibly due to Gee-radical scavenging activity of plant.

Objective:To evaluate wound healing potential of flavonoid fractions of Martynia annua (M.annua) Linn,leaves in diabetic rats on the basis of folkloric information and preliminary study.Methods:The flavonoid compound luteolin and apigenin were isolated from dried leaves of plant by column chromatography.The two concenlrations(0.2%and 0.5%w/w) of luteolin and flavonoid fraction were selected for topically applied as ointment on diabetic wound. The Povidone Iodine Ointment USP was used as a reference.On 18th days,protein content, hydroxyproline and antioxidants(SOD,CAT and GSH) level in granuloma tissues were determined. Results:The results showed that,percent wound contraction were observed significantly(P<0.01) greater in MAF fraction and 0.5%w/w of luteolin treatment groups.Presence of matured collagen fibres and fibroblasts with better angiogenesis were observed in histopathological studies. Conclusions:In conclusion,our findings suggest that flavonoid fraction(MAF) and luteolin(0.5% w/w) may have potential benefit in enhancing wound healing in diabetic condition,possibly due to Gee-radical scavenging activity of plant.