摘要
Objective:To investigate the antioxidant and hepatoproteetive activity of methanolic flower extract of Nerium oleander against CCl<sub>4</sub>—induced hepatotoxicity in rats.Methods:In vitro antioxidant activity of methanolic extract of dowers of Nerium oleander（MENO-F） was evaluated by various assays,including reducing power,lipid peroxidation.DPPH.ARTS,superoxide anion,hydroxyl radicals and metal chelation.The hepatoproteetive and in vivo antioxidant activity of MENO-F were evaluated against CCl<sub>4</sub>-induced hepatic damage in rats.The MENO-F at dose of 100.200 and 400 mg/kg were administered orally once daily for seven days.Serum enzymatic levels of serum glutamate oxaloacetate transaminase（AST）,serum glutamate pyruvate transaminase（ALT）,serum alkaline phosphatase（ALP） and total bilirubin were estimated along with estimation of superoxide dismutase（SOD） and malondialdehvde（MDA） levels in liver tissues.Further histopathological examination of the liver sections was carried out to support the induction of hepalotoxicity and hepatoproteetive efficacy.Results:The extract showed potent activities on reducing power,lipid peroxide.DPPH.ABTS.superoxide anion,hydroxyl radical and metal chelation.The substantially elevated serum enzymatic levels of AST,ALT.ALP and total bilirubin were found to he restored towards normalization significantly by the MENO-F in a dose dependent manner with maximum hepatoprotection at 400 mg/kg dose level.The histopathological observations supported the biochemical evidences of hepatoprotection.Elevated level of SOD and decreased level of MDA further strengthen the hepatoproteetive observations. The results of the present study strongly reveal that MENO-F has potent antioxidant activity and hepatoproteetive activity against CCl<sub>4</sub>—induced hepatic damage in experimental animals.
Objective:To investigate the antioxidant and hepatoproteetive activity of methanolic flower extract of Nerium oleander against CCl_4—induced hepatotoxicity in rats.Methods:In vitro antioxidant activity of methanolic extract of dowers of Nerium oleander(MENO-F) was evaluated by various assays,including reducing power,lipid peroxidation.DPPH.ARTS,superoxide anion,hydroxyl radicals and metal chelation.The hepatoproteetive and in vivo antioxidant activity of MENO-F were evaluated against CCl_4-induced hepatic damage in rats.The MENO-F at dose of 100.200 and 400 mg/kg were administered orally once daily for seven days.Serum enzymatic levels of serum glutamate oxaloacetate transaminase(AST),serum glutamate pyruvate transaminase(ALT),serum alkaline phosphatase(ALP) and total bilirubin were estimated along with estimation of superoxide dismutase(SOD) and malondialdehvde(MDA) levels in liver tissues.Further histopathological examination of the liver sections was carried out to support the induction of hepalotoxicity and hepatoproteetive efficacy.Results:The extract showed potent activities on reducing power,lipid peroxide.DPPH.ABTS.superoxide anion,hydroxyl radical and metal chelation.The substantially elevated serum enzymatic levels of AST,ALT.ALP and total bilirubin were found to he restored towards normalization significantly by the MENO-F in a dose dependent manner with maximum hepatoprotection at 400 mg/kg dose level.The histopathological observations supported the biochemical evidences of hepatoprotection.Elevated level of SOD and decreased level of MDA further strengthen the hepatoproteetive observations. The results of the present study strongly reveal that MENO-F has potent antioxidant activity and hepatoproteetive activity against CCl_4—induced hepatic damage in experimental animals.
