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The extract of Mucuna pruriens possesses histamine activity

The extract of Mucuna pruriens possesses histamine activity
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摘要 Objective:To investigate the activity of the active principle in the spines of the seed pods of Mucuna pruriens using contraction of guinea pig ileum as index of pharmacological activity. Methods:The active principle was extracted with 0.0015 M NaCl.Muscle strips of guinea pig ileum were prepared and contractile responses were measured using a Kymograph.Two sets of experiment were conducted:(1).The contraction of the ileum in presence of different concentrations of histamine,2-methylhistamine and the extract of Mucuna pruriens.(2).The contractile response of the ileum in presence of different concentrations of the extract and antagonists including diphenhydramine,atropine and methysergide.Results:(1) The extract of Mucuna pruriens hair, 2- methylhistamine and histamine produced dose dependent contraction of guinea pig ileum (Extract ED<sub>50</sub> = 13.0μg/mL,2-methylhistamine ED<sub>50</sub>=8.5μg/mL and histamine ED<sub>50</sub>=10.0μg/ mL).(2) Diphenhydramine,an H,antagonist competitively blocked the contractile response of the Mucuna pruriens extract(3) Coadminstration of the Mucuna pruriens extract either with different doses of antimuscarinic agent atropine or 5-hydroxytryptamine blocking agent methysergide did not alter the extract induced contractile response of the guinea pig ileum.Conclusion:These results demonstrate that the spines of Mucuna pruriens possess histamine activity which may contribute to its itching and painful irritation effects. Objective:To investigate the activity of the active principle in the spines of the seed pods of Mucuna pruriens using contraction of guinea pig ileum as index of pharmacological activity. Methods:The active principle was extracted with 0.0015 M NaCl.Muscle strips of guinea pig ileum were prepared and contractile responses were measured using a Kymograph.Two sets of experiment were conducted:(1).The contraction of the ileum in presence of different concentrations of histamine,2-methylhistamine and the extract of Mucuna pruriens.(2).The contractile response of the ileum in presence of different concentrations of the extract and antagonists including diphenhydramine,atropine and methysergide.Results:(1) The extract of Mucuna pruriens hair, 2- methylhistamine and histamine produced dose dependent contraction of guinea pig ileum (Extract ED_(50) = 13.0μg/mL,2-methylhistamine ED_(50)=8.5μg/mL and histamine ED_(50)=10.0μg/ mL).(2) Diphenhydramine,an H,antagonist competitively blocked the contractile response of the Mucuna pruriens extract(3) Coadminstration of the Mucuna pruriens extract either with different doses of antimuscarinic agent atropine or 5-hydroxytryptamine blocking agent methysergide did not alter the extract induced contractile response of the guinea pig ileum.Conclusion:These results demonstrate that the spines of Mucuna pruriens possess histamine activity which may contribute to its itching and painful irritation effects.
出处 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第1期29-31,共3页 亚太热带医药杂志(英文版)
关键词 MUCUNA pruriens HISTAMINE ACTIVITY ITCHING IRRITATION Mucuna pruriens Histamine activity Itching Irritation
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参考文献7

  • 1Erspamer,V. Handbuch d. exp. Pharmakologie, Vol. XIX, 5-hydroxytryptamine and related indolealkylamines . 1966
  • 2Lorenz,W.,Doenicke,A.,Schoning,B.,Neugebauer,E.,Thornton.The role of histamine in adverse reactions to intravenous agents[].Adverse Reactions to Anaesthetic Drugs.1981
  • 3Lewis,W.H.,Elvin-Lewis,P.F. Medical Botany . 1977
  • 4Hutchinson J,Dalziel JM.Crown agents for oversea government[].Flora of west tropical Africa.1963
  • 5Weck ALD,Bundgaard H.Alergic reactions to drugs[].Hand book of experimental pharmacology.1983
  • 6Essman WB.Serotonin distribution in tissues and fluids[].Serotonin in health and disease.1978
  • 7Lampe KF,Fagerstrom R.Plant toxicity and dermatitis[]..1986

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