摘要
Objective:To investigate the role and mechanism of tamarind seed coat extract(TSCE) on normal human skin fibroblast CCD-l064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide(H_2O_2).Methods:Tamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell analysis.Effect of TSCE on intracellular reactive oxygen species(ROS),glutathione(GSH) level,antioxidant enzymes such as superoxide dismulase(SOU),glutathione peroxidase(CPx) and catalase activity including antioxidant protein expression was investigated.Results:TSCE significantly attenuated intracellular ROS in the absence and presence of H_1O_2by increasing GSH level In the absence of H_2O_2,TSCE significantly enhanced SOD and catalase activity but did not affected on CPx.Meanwhile,TSCE significantly increased the protein expression of SOD and CPx in H_2O_2-treated cells.Conclusions:TSCE exhibited antioxidant activities by scavenging ROS.attenuating GSll level that could protect human skin fibroblast cells from oxidative stress.Our results highlight the antioxidant mechanism of tamarind seed coat through an antioxidant enzyme system,the exlracl potentially benefits for health food and cosmeceutical application of tamarind seed coat.
Objective:To investigate the role and mechanism of tamarind seed coat extract（TSCE） on normal human skin fibroblast CCD-l064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide（H2O2）.Methods:Tamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell analysis.Effect of TSCE on intracellular reactive oxygen species（ROS）,glutathione（GSH） level,antioxidant enzymes such as superoxide dismulase（SOU）,glutathione peroxidase（CPx） and catalase activity including antioxidant protein expression was investigated.Results:TSCE significantly attenuated intracellular ROS in the absence and presence of H1O2by increasing GSH level In the absence of H2O2,TSCE significantly enhanced SOD and catalase activity but did not affected on CPx.Meanwhile,TSCE significantly increased the protein expression of SOD and CPx in H2O2-treated cells.Conclusions:TSCE exhibited antioxidant activities by scavenging ROS.attenuating GSll level that could protect human skin fibroblast cells from oxidative stress.Our results highlight the antioxidant mechanism of tamarind seed coat through an antioxidant enzyme system,the exlracl potentially benefits for health food and cosmeceutical application of tamarind seed coat.
基金
Supported by a grant under the Strategic Scholarships for Frontier Research Network program(Grant No.9/2551)
the 90th Anniversary of Chulalongkorn University Fund.Chulalongkorn University,Thailand,(Grant No.1/26)
