Antioxidant,antibacterial and cytotoxic effects of the phytochemicals of whole Leucas aspera extract

Objective:To investigate the antioxidant,antibacterial and cytotoxic activity of whole Leucas aspera(Labiatae)(L.aspera)alcoholic extract.Methods:Whole L.aspera powder was extracted by absolute ethanol(99.50%).The ethanolic extract was subjected to antioxidant,antibacterial and brine shrimp lethality assay.Results:The extract showed potent radical scavenging effect(antioxidant)with IC_(50)value of(99.58±1.22)μg/mL which was significant(P<0.01)in comparison to ascorbic acid with IC_(50)value of(1.25±0.95)μg/mL.In case of antibacterial screening,the extract showed notable antibacterial effect against the tested microbial strains.Significant(P<0.05)zone of inhibitions against Gram positive Bacillus subtilis[(12.00±1.32)mm]and Bacillus megaterium[(13.00±1.50)mm],Staphylococcus aureus[(8.00±0.50)mm]and Gram negative Salmonella typhi[(6.00±0.50)mm],Salmonella paratyphi[(8.00±1.00)mm],Shigella dysenteriae[(9.00±1.32)mm]and Vibrio cholerae[(9.00±0.66)mm]was observed.In brine shrimp lethality bioassay,the extract showed the LC_(50)value as(181.68±2.15)μg/mL which was statistically significant(P<0.01)compared to positive control vincristine sulfate[LC_(50)=(0.76±0.04)μg/mL].Conclusions:The results demonstrate that the ethanolic extract of L.aspera could be used as antibacterial,pesticidal and various pharmacologic actives.

Objective:To investigate the antioxidant,antibacterial and cytotoxic activity of whole Leucas aspera（Labiatae）（L.aspera）alcoholic extract.Methods:Whole L.aspera powder was extracted by absolute ethanol（99.50%）.The ethanolic extract was subjected to antioxidant,antibacterial and brine shrimp lethality assay.Results:The extract showed potent radical scavenging effect（antioxidant）with IC50value of（99.58±1.22）μg/mL which was significant（P&lt;0.01）in comparison to ascorbic acid with IC50value of（1.25±0.95）μg/mL.In case of antibacterial screening,the extract showed notable antibacterial effect against the tested microbial strains.Significant（P&lt;0.05）zone of inhibitions against Gram positive Bacillus subtilis[（12.00±1.32）mm]and Bacillus megaterium[（13.00±1.50）mm],Staphylococcus aureus[（8.00±0.50）mm]and Gram negative Salmonella typhi[（6.00±0.50）mm],Salmonella paratyphi[（8.00±1.00）mm],Shigella dysenteriae[（9.00±1.32）mm]and Vibrio cholerae[（9.00±0.66）mm]was observed.In brine shrimp lethality bioassay,the extract showed the LC50value as（181.68±2.15）μg/mL which was statistically significant（P&lt;0.01）compared to positive control vincristine sulfate[LC50=（0.76±0.04）μg/mL].Conclusions:The results demonstrate that the ethanolic extract of L.aspera could be used as antibacterial,pesticidal and various pharmacologic actives.