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Expression of dectin-1 during fungus infection in human corneal epithelial cells 被引量：6

Expression of dectin-1 during fungus infection in human corneal epithelial cells
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摘要 AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1(dectin-1) in human corneal epithelial(HCE) cells infected by fungus. · METHODS: A total of 20 cases of healthy donor corneas were group A,and 20 patients(20 eyes) suffered from fungal keratitis(FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus(AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0,4,8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately. ·RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h,and dectin-1 protein expression increased after stimulation at 24h. · CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro. AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1(dectin-1) in human corneal epithelial(HCE) cells infected by fungus. · METHODS: A total of 20 cases of healthy donor corneas were group A,and 20 patients(20 eyes) suffered from fungal keratitis(FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus(AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0,4,8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately. ·RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h,and dectin-1 protein expression increased after stimulation at 24h. · CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro.
作者 Cui Li Gui-Qiu Zhao Cheng-Ye Che Na Li Jing Lin Qiang Xu Qian Wang Ying Liu Sheng Qiu
机构地区 Department of Ophthalmology
出处 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2014年第1期34-37,共4页 国际眼科杂志（英文版）
基金 Supported by National Natural Science Foundation of China(No.81170825 No.81300730)
关键词 DECTIN-1 corneal epithelial cells fungal keratitis HUMAN dectin-1, corneal epithelial cells, fungal keratitis, human
分类号 R772.21 [医药卫生—眼科]
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参考文献3

  • 1Leal, Sixto M,Vareechon, Chairut,Cowden, Susan,Cobb, Brian A,Latgé, Jean-Paul,Momany, Michelle,Pearlman, Eric.Fungal antioxidant pathways promote survival against neutrophils during infection[J].EN.2012(7)
  • 2Hye-Mi Lee,Jae-Min Yuk,Dong-Min Shin,Eun-Kyeong Jo.Dectin-1 is Inducible and Plays an Essential Role for Mycobacteria-Induced Innate Immune Responses in Airway Epithelial Cells[J].Journal of Clinical Immunology.2009(6)
  • 3Lixin Xie,Wenxian Zhong,Weiyun Shi,Shiying Sun.Spectrum of Fungal Keratitis in North China[J].Ophthalmology.2006(11)

同被引文献56

  • 1Cheng-Ye Che,Cui Li,Ang Gao,Jing Lin,Li-Li Zhang,Qiang Xu,Qian Wang,Gui-Qiu Zhao.Dectin-1 expression at early period of Aspergillus fumigatus infection in rat's corneal epithelium[J].International Journal of Ophthalmology(English edition),2013,6(1):30-33. 被引量：4
  • 2Cheng-Ye Che,Wen-Yan Jia,Qiang Xu,Na Li,Li-Ting Hu,Nan Jiang,Jing Lin,Qing Wang,and Gui-Qiu Zhao.The roles of surfactant protein D during Aspergillus fumigatus infection in human corneal epithelial cells[J].International Journal of Ophthalmology(English edition),2012,5(1):13-17. 被引量：13
  • 3高建鲁,吴欣怡.人角膜上皮细胞Toll样受体介导的炎性细胞因子的表达[J].中华眼科杂志,2006,42(7):628-633. 被引量：15
  • 4Sixto M. Leal,Eric Pearlman.The role of cytokines and pathogen recognition molecules in fungal keratitis – Insights from human disease and animal models[J]. Cytokine . 2012 (1)
  • 5Leal Sixto M,Cowden Susan,Hsia Yen-Cheng,Ghannoum Mahmoud A,Momany Michelle,Pearlman Eric.Distinct roles for Dectin-1 and TLR4 in the pathogenesis of Aspergillus fumigatus keratitis. PLoS pathogens . 2010
  • 6Dominikus Strasser,Konstantin Neumann,Hanna Bergmann,Mohlopheni J. Marakalala,Reto Guler,Anna Rojowska,Karl-Peter Hopfner,Frank Brombacher,Henning Urlaub,Gottfried Baier,Gordon D. Brown,Michael Leitges,Jürgen Ruland.Syk Kinase-Coupled C-type Lectin Receptors Engage Protein Kinase C-δ to Elicit Card9 Adaptor-Mediated Innate Immunity[J]. Immunity . 2012 (1)
  • 7J. Y.Hong,K. E.Lee,K. W.Kim,M. H.Sohn,K.‐E.Kim.Chitinase Induce the Release of IL‐8 in Human Airway Epithelial Cells, Via Ca<sup>2+</sup>‐dependent PKC and ERK Pathways[J]. Scandinavian Journal of Immunology . 2010 (1)
  • 8Wei-Ning Lin,Shue-Fen Luo,Chih-Chung Lin,Li-Der Hsiao,Chuen-Mao Yang.Differential involvement of PKC-dependent MAPKs activation in lipopolysaccharide-induced AP-1 expression in human tracheal smooth muscle cells[J]. Cellular Signalling . 2009 (9)
  • 9Ji-Sook Lee,In Sik Kim,Ji-Sun Ryu,Chi-Young Yun.House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, IL-6, and IL-8 in human monocytic THP-1 cells[J]. Cytokine . 2008 (3)
  • 10Edward C. Dempsey,Carlyne D. Cool,Cassana M. Littler.Lung disease and PKCs[J]. Pharmacological Research . 2007 (6)

引证文献6

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International Journal of Ophthalmology(English edition)
2014年 第1期

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