摘要
AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six groups.Group A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of prematurity.The rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after P12.The retinas were whole-mounted and imaged with a confocal microscopy.The vascular branching points were counted to quantify neovascularization at P17.Cross-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular response.RESULTS:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were observed.Sorafenib-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P【0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,respectively.The number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,respectively.There were significant difference between each group(P【0.05)except groups B and C.CONCLUSION:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.
AIM:To investigate the effect of intravitreal injection administered sorafenib,a multikinase inhibitor,in a rat model of oxygen-induced retinopathy(OIR).METHODS:Seven-day-old Sprague-Dawley rats(n=144)were randomly assigned to six groups.Group A received normal partial oxygen pressure and groups B,C,D,E and F were exposed to hyperoxia(75±2)%from postnatal 7d(P7)to P12 to induce retinopathy of prematurity.The rats in groups C,D,E and F were received intravitreal injections of either vehicle(DMSO)or sorafenib at P12(5,20 and 80μg,respectively).Then they returned to normoxia after P12.The retinas were whole-mounted and imaged with a confocal microscopy.The vascular branching points were counted to quantify neovascularization at P17.Cross-sections of the retina were stained with hematoxylin and eosin(HE).The nuclei of new vessels breaking the internal limiting membrane were counted to quantify the proliferative neovascular response.RESULTS:The retinal vessel in groups B and C turned into tortuosity and a great deal of neovascularization were observed.Sorafenib-treated rats had significantly less neovascularization as compared with vehicle-treated and control rats in a dose dependent manner(P<0.05).The number of vascular branching points in A,B,C,D,E and F were 16.50±3.90,37.44±6.47,37.08±5.10,30.80±6.85,26.08±5.08 and 19.83±3.51,respectively.The number of the nuclei of retinal new vessel in A,B,C,D,E and F were 0.22±0.42,35.66±4.70,35.30±4.54,27.30±4.28,21.41±3.53,and 7.41±2.87,respectively.There were significant difference between each group(P<0.05)except groups B and C.CONCLUSION:In the rat OIR model,sorafenib could inhibit retinal neovascularization in a dose dependent manner.
