青鳞鱼蛋白复合酶控制水解动力学模型的研究

Hydrolysis Dynamics Model of Protein from Harengula zunasi by Compound Enzymes

在假设复合酶恒温控制水解动力学遵循内切酶限制水解动力学历程的前提下 ,采用实验方法求出了复合酶恒温控制水解动力学模型。结果表明 ,复合酶对青鳞鱼蛋白进行控制水解的动力学模型为 :R =(0 .315 4e0 - 0 .0 186s0 )exp(- 0 .170DH) ,DH =5 .882ln[1+(0 .0 5 36 2e0 /s0 -0 .0 0 32 )t];其酶失活常数Kd=0 .0 5 36min- 1;水解反应能够顺利进行的条件是 :e0 /s0 >c0 ,常数c0 =5 .90× 10 - 2 。验证实验证明 ,根据复合酶恒温控制水解动力学模型得到的理论DH与实际DH基本吻合 ,该动力学模型具有很强的实用价值。

In this study, we supposed that hydrolysis dynamics model was controlled by compound enzymes at the same temperature followed the course of limit hydrolysis dynamics model of inner-enzyme, thus obtained hydrolysis dynamics model controlled by compound enzymes at the same temperature. These results suggested that the hydrolysis dynamics model controlled by compound enzymes of protein from Hargenlua zunasi expressed as:R=(0 315 4 e 0- 0.018 6 s 0)exp(-0.170 DH),DH=5.882 ln\[1+(0.053 62 e 0/s 0-0.003 2)t\]; the inactive constant of enzyme is K d=0.053 6 min -1; and the optimum conditions that assure hydrolysis successfully is :e 0/s 0>c 0, the constant c 0=5.90×10 -2. The experiment tested that, because the theoretical DH of hydrolysis dynamics model controlled by compounds is consistent with the practical DH, therefore the dynamics model has high applied value.