依折麦布联合辛伐他汀对于ApoE-/-小鼠动脉粥样硬化的影响

Effects of Coadministration of Simvastatin with Ezetimibe on Atherosclerosis in ApoE-/-Mice

目的：探讨辛伐他汀联合依折麦布对动脉粥样硬化的ApoE-/-小鼠血清炎性因子的影响。方法高脂饲料喂养ApoE-/-小鼠，建立动脉粥样硬化模型。将动脉粥样硬化的ApoE-/-小鼠30只随机分为3组。模型组不给药；单药治疗组给予辛伐他汀片20 mg?kg-1?d-1；联合治疗组给予辛伐他汀20 mg?kg-1?d-1及依折麦布片5 mg?kg-1?d-1，野生的普通C57小鼠10只作为对照组。给药4周后，酶法检测血清HDL-C、LDL-C、总胆固醇及甘油三酯。ELISA法检测各组血清TNF-α及MCP-1含量。结果与单药治疗组比较，联合治疗组更有效降低总胆固醇、甘油三酯、LDL-C，更显著降低TNF-α及MCP-1，差异有统计学意义。结论依折麦布与辛伐他汀联用较单用辛伐他汀可强化降脂、减轻炎症反应，发挥抗动脉粥样硬化作用。

Objective To investigate the effects of coadministration of Atorvastatin with Ezetimibe on serum inflammatory factors in atherosclerosis ApoE-/-mice. Methods Thirty ApoE-/-mice were treated with high cholesterol diet 8 weeks to set up atherosclerosis model. Then, thirty ApoE-/-atherosclerotic mice were randomly divided into 3 groups:model group (n=10), Simvastatin treatment group (n=10), and coadministration group (n=10) and respectively treated with no drug, Simvastatin 20 mg?kg-1?d-1, and Simvastatin 20 mg?kg-1?d-1+Ezetimibe 5 mg?kg-1?d-1 for 4 weeks. 10 common wild C57 mice served as control group. Serum lipid level(TG, TC, HDL-C and LDL-C) was measured by enzymatic method. Serum TNF-αand MCP-1 level were measured by ELISA. Results Compared with simvastatin treatment group, the coadministration of Ezetimibe could better decrease the level of serum TC, TG, LDL-C, TNF-αand MCP-1. There was significant difference between Simvastatin group and coadministration group. Conclusion The coadministration of Simvastain and Ezetimibe may play a good role in anti-atherosclerosis by decreasing the level of serum TC, TG and LDL-C and reducing the inflammation reaction.