CXC趋化因子配体5对前列腺癌细胞生长的作用

The effect of CXCL5 on the growth of prostate cancer cells

目的研究CXC趋化因子配体(CXCL)5受体(CXCR2)蛋白在前列腺癌细胞株PC-3、DU145和LNCaP细胞中的表达情况及CXCL5对LNCaP细胞生长的影响。方法应用Western印迹技术检测CXCR2在3种前列腺癌细胞株PC-3、DU145和LNCaP细胞中的表达情况,筛选出表达量最高的细胞株,然后用MTT法检测5、10、20 ng/ml浓度CXCL5对CXCR2蛋白表达量最高的前列腺癌细胞生长的影响。结果在PC-3、DU145和LNCaP细胞中均有CXCR2蛋白的表达,LNCaP细胞中最高(1.65±0.089),DU145细胞次之(1.44±0.107),PC-3细胞中最低(0.36±0.056)(P<0.05);5、10、20 ng/ml CXCL5浓度组作用LNCaP细胞6 d后,吸光度值分别为0.55±0.06,0.61±0.08和0.73±0.10,与对照组(0.52±0.14)比较,20 ng/ml CXCL5浓度组增长率明显增高(P<0.05)。结论 CXCL5可通过作用于其自身受体CXCR2进而促进LNCaP细胞生长。

Objective To study the expression of the receptor CXCR 2 of CXCL5 in prostate cancer cell lines PC-3, DU145, LNCaP and the effect of CXCL5 to the growth of LNCaP prostate cancer cell line .Methods The expression of CXCR2 in three prostate cancer lines PC-3, DU145 and LNCaP were measured by Western blot .The highest expression cell line was screened , and the effect of 5, 10 and 20 ng/ml CXCL5 on the growth of the highest expression cell line was detected by MTT .Results The proteins of CXCR2 were expressed in PC-3, DU145 and LNCaP cell lines.The expressions of CXCR2 from high-level to low-level were LNCaP cell line(1.65±0.089), DU145 cell line(1.44±0.107)and PC-3 cell line (0.36±0.056)(P<0.05);After 5,10 and 20 ng/ml CXCL5 acted on LNCaP cell line for 6 days, the absorbance values were 0.55 ±0.06 ,0.61 ±0.08 and 0.73 ±0.10 , respectively .Compared with that in control group , 20 ng/ml CXCL5 significantly increased growth rate of LNCaP cell line .Conclusions CXCL5 can promote growth of LNCaP cell line by acting on autoreceptor .