丹参联合小剂量泼尼松龙对口腔黏膜下纤维性变相关miRNA表达的改变

Changes of miRNA after oral submucous i brosis co-cultured with Salvia and low-dose prednisolone

目的:探讨与分析丹参联合小剂量泼尼松龙对口腔黏膜下纤维性变(oral submucous fibrosis,OSF)相关miRNA表达的改变。方法:选取临床上具有典型中、晚期病理特征的OSF组织及正常口腔颊黏膜配对组织各10对。对正常和病变组织行昂飞miRNA芯片筛查,发现与OSF相关的miRNA表达谱。从正常口腔黏膜组织获得原代成纤维细胞,以槟榔碱(50μg/mL)诱导培养3,6,12 d后(以0 d为对照),检测差异miRNA的表达。原代培养OSF的成纤维细胞,经丹参(90 mg/mL)联合小剂量泼尼松共培养12,24,36 h后(以0 h为对照),检测差异miRNA的表达。结果:槟榔碱能诱导正常口腔黏膜成纤维细胞中miRNA的表达改变,而丹参联合小剂量泼尼松龙能逆转OSF成纤维细胞中相关miRNA的表达。结论:临床组织验证提示差异miRNA在OSF的发生、发展中发挥作用,丹参联合小剂量泼尼松龙能逆转相关miRNA的表达。

Objective: To explore and analy ze the the expression change of miRNA associated with oral submucous fibrosis(OSF) treated by the Salvia combined with law-dose prednisolone. Methods: Ten pairs of tissues from patients with typical early or advanced stage clinical pathological features of OSF and their paired normal tissues(internal control), were selected respectively. The miRNA expression profiles between the OSF and its pairedcontrols were compared by the Affymetrix analysis. The primary normal oral mucous cells were cultured in arecoline(50 μg/mL) for 3, 6, 1 2 d( 0 d s e r ved a s co n t rol), and the primary OSF-fibroblast cells were cultured with Salvia(90 mg/mL) combined with low-dose prednisolone for 12, 24, 36 h(0 h served as control). The differential expression of miRNA was detected. Results: Arecoline induced the expression changes of miRNAs in normal mucosal cells. Salvia combined with low doses of prednisolone reversed the related miRNA expression. Conclusion: MiRNAs play an essential role in the occurrence and development of OSF. Salvia combined with low-dose prednisolone can reverse the expression of related miRNAs in OSF cells.