复合阿伦磷酸钠骨水泥对成骨细胞的影响

Effect of alendronate-loaded bone cement on osteoblast

目的:研究不同阿伦磷酸钠含量的骨水泥对成骨细胞的影响,确定复合阿伦磷酸钠骨水泥的细胞毒性.方法:根据阿伦磷酸钠含量不同分成6组,在50 g Cemex*XL骨水泥粉末中分别加入0,10,50,100,500,1 000 mg阿伦磷酸钠,依次命名为G0～G5组.通过电镜观察各组成骨细胞样MG-63的黏附能力,MTT比色法测定吸光度值,碱性磷酸酶试剂盒测定碱性磷酸酶(alkaline phosphatase activity,AKP)活性,Annexin-V-FITC凋亡检测试剂盒测定凋亡率,相差显微镜观察骨矿化情况.结果:各组MG-63细胞黏附能力良好;与Go组相比,G1～G4组抑制细胞凋亡,G5组促进细胞凋亡和抑制细胞增殖(p＜0.05);各组特异性碱性磷酸酶活性、骨矿化能力差异无统计学意义(p＞0.05).结论:在50 g骨水泥粉末中加入阿伦磷酸钠不超过500 mg对成骨细胞无毒性.

Objective: To determine the effect of bone cement, with different amounts of alendronate on osteoblast, and determine the cytotoxicity of alendronate-integrated bone cement from the viewpoint of cell biology. Methods: According to dif erent additions(0, 10, 50, 100, 500, 1 000 mg) of alendronate in 50 g Cemex?XL bone cement powder, the experiments were divided into 6 groups, namely G0–G5 groups. In all groups, the adhesive capacity of osteoblast-like cells MG-63 was evaluated by electron microscope, the optical density(OD) value of cells by MTT colorimetry method, the alkaline phosphatase activity(AKP) by AKP assay kit, the apoptosis rates by Annexin-V-FITC apoptosis detection kit, and the bone mineralization potentiality by phase contrast microscope. Results: h e adhesive capacity of MG-63 was good in all groups. Compared with the G0 group, the cell apoptosis was inhibited in G1–G4 groups while in G5 group the cell apoptosis was promoted and cell proliferation was inhibited(P<0.05). In all groups, no signii cant dif erence was found in alkaline phosphatase activity and bone mineralization potentiality(P>0.05).Conclusion: Less than 500 mg alendronate added in Cemex?XL 50 g bone cement powder has no cytotoxicity on osteoblasts.