内皮细胞促进胰岛的成活与功能

Endothelial cells promote islet survival and function

目的:探讨内皮细胞对胰岛细胞活性与功能的支持作用。方法:内皮细胞和胰岛分别被分离纯化,在共培养中评估胰岛细胞活性与功能;体内试验分为单纯胰岛移植组、胰岛-内皮共移植组、内皮细胞移植组和PBS对照组,测定血糖和胰岛素水平,免疫组织化学染色及电镜下观察组织细胞形态、结构及细胞标志物鉴定。结果:培养7 d,超过90%的胰岛细胞显示正常形态。共培养组中的胰岛素释放水平显著高于胰岛培养组(P<0.05)。移植3 d后,各移植组的血糖和胰岛素水平明显不同(P<0.05)。胰岛-内皮共移植组和单纯移植组间比较,胰岛存活时间差异有统计学意义(P=0.001)。结论:与内皮细胞在体外共培养能改善分离的大鼠胰岛细胞活性与功能,同内皮细胞共移植能够有效地延长糖尿病大鼠胰岛移植物存活期。

Objective: To investigate islet grat survival and function at er co-culture and co-transplantation with vascular endothelial cells(ECs) in diabetic rats. Methods: We isolated ECs, and assessed the viability of isolated islets in a group of standard culture and a group of co-culture with ECs. h en we put the diabetic rats in 4 groups: an islet transplantation group, an islet grat with EC transplantation group, an EC transplantation group, and a PBS control group. Blood glucose and insulin concentrations were measured daily. Cell morphology and cell markers were investigated by immunohistochemical staining and electron microscope. Results: Normal morphology was shown in more than 90% of AO/PI staining positive islets while co-cultured with ECs for 7 days. Insulin release assays showed a significantly higher simulation index co-culture except for the first day(P<0.05). There was a significant difference in concentrations of blood glucose and insulin among the 4 groups after 3 days after thetransplantation(P<0.05). Conclusion: EC-islet co-culture can improve the function and survival of isolated islets in vitro, and EC-islet co-transplantation can ef ectively prolong the islet grat survival in diabetic rats.