大强度训练后机体免疫机能的变化及其和垂体—肾上腺皮质系统的可能关系

Changes in T-Lymphocyte Subset After High Intense Training and Their Possible Relationship With the Changes in ACTH and GC

本文研究四周大强度训练对运动员外周血T淋巴细胞及其亚群的影响及其和垂体—肾上腺皮质系统的可能关系。采用双色免疫荧光法流式细胞仪分析T淋巴细胞及其亚群,放射免疫分析法测定血浆ACTH和GC浓度。四周训练前后自身比较的结果表明,大强度训练引起CD8+细胞百分比升高,结果CD4/CD8比值下降,出现倒置现象,而血浆ACTH和GC浓度分别较四周前提高75%和96%。结果提示,过量训练所致的抑制性T淋巴细胞的过渡激活继发造成免疫抑制。在考虑增加训练负荷时必须以机体免疫力得到恢复为前提,避免出现过度训练综合症,达到提高运动能力的目的。

Peripheral blood T-lymphocyte subset and ACTH and GC concentration in plasma in middle-and long-distance runners were measured simultaneously to study the changes in peripheral blood T-lymphocyte subset and their possible relationship with the changes in plasma ACTH and GC concentration.Lymphocyte phenotype was detected by flow cytometry and ACTH and GC concentration were measured by radioimmunoassay.The results showed that after four weeks high intense training the percentage of CD_8^+ cells was significantly increased,the ration of CD_4/ CD_8 was significantly lower in post-training than in pre-training,while the concentration of plasma ACTH and GC was 75% and 96% higher than pre-training.Possible relationship between the changes in T-lymphocyte subset and ACTH and GC concentration and its physiological significance were also discussed.