摘要
为了研究肾脏 EPO代谢在运动性贫血发生机理中的功能和作用 ,及营养补充对运动性贫血防治效果的作用机制 ,对 30只雄性 Wistar大鼠进行等量随机分组 :对照组 (C)、运动组 (P)和运动 +营养组 (G)。11周的跑台运动和营养补充结束后 ,应用 RT- PCR和 EL ISA方法测试大鼠肾脏 EPO基因表达和活性水平。结果发现 ,肾脏组织匀浆液 EPO水平并未表现出对照组、运动 +营养组、运动组的组间差异 ,而对于肾脏 EPO m RNA表达指标在运动组分别显著高于对照组和运动 +营养组 ,提示肾脏 EPO活性水平可能并非机体运动性贫血的发病因素。
In order to investigate the possible role of kidney EPO metabolism in the development of exercise anemia,30 male Wistar rats are randomly assigned to three groups:control(C,n=10),exercise(P,n=10),exercise +nutrition(G,n=10).At the end of eleventh week of treadmill and nutrition supplementation,gene expression and activity of kidney EPO in rats are measured with RT-PCR and ELISA method.The result shows that there is no significant change in EPO activity.However,gene expression level of EPO in P group is higher than that of C and G groups respectively.It suggests that EPO activity of kidney probably is not the mechanism of exercise anemia.
出处
《体育科学》
CSSCI
北大核心
2004年第8期26-29,共4页
China Sport Science
基金
国家自然科学基金资助项目 (3 0 2 70 64 2 )
国家科技部奥运攻关基金资助项目 (2 0 0 2 BA90 4B0 4-4 )
