β-淀粉样肽鼠脑室内灌注制作阿尔茨海默病动物模型的实验技术研究

β-amyloid intraventricular infusion for rodent models ofAlzheimer disease

目的 探讨 β -淀粉样肽鼠脑室内灌注制作阿尔茨海默病 (AD)模型过程中的技术问题。 方法 经颅骨穿刺进入鼠侧脑室 ,以微渗透泵持续灌注 β -淀粉样肽 (Aβ) ,使Aβ弥散入脑室周围及皮层 ,造成与神经元相关的Aβ沉积等类似AD的病理变化。 4周后鼠脑冠状位连续切片 ,在普通光学显微镜下观察脑导管进入侧脑室的痕迹。结果 大鼠有 92 % (5 5 /6 0 )导管准确进入侧脑室 ,接近进入或在皮层 5 % (3/6 0 ) ,小鼠仅 4 3% (2 3/5 4 )导管进入侧脑室 ,接近进入或在皮层 31% (17/5 4 )。结论 大鼠脑室内灌注Aβ的方法可作为AD动物模型的方法之一。

Objective To investigate the techniques of Aβ intraventricular infusion for rodent models of Alzheimer disease. Methods A brain catheter was placedin lateral ventricle through a hole drilled in skull and a miniosmotic pump containing Aβ was attached via polyethylene tube to brain catheter in rats (n=60) and mice (n=54), then Aβ was infused into the ventricle and diffuseddeposition in cortex and circumventriclar regions. After 4 weeks, animal brainswere coronally sectioned consecutively and observed the needle track of the catheter under light microscope. Results The catheters were properly implantedinto the ventricle in 92%(55/60) of rats and in 43%(23/54) of mice and implanted close to the ventricle in 5 %( 3/60) of rats and in 31%(17/54) of mice. Conclusion Intraventricular infusion of Aβ in rat is an approach to Alzheimer disease models.