和营安心方对过氧化氢损伤大鼠胚胎心肌细胞的保护作用

Protective Effects of He Ying An Xin Fang on Free Radical Injury Induced by H_2O_2 in Myocardium Rats

目的:探讨和营安心方对过氧化氢(hydrogen peroxide,H2O2)所致大鼠胚胎心肌细胞损伤的保护作用。方法:建立H2O2致大鼠胚胎心肌细胞氧化应激损伤模型,设正常对照组(Control)、过氧化氢(200μmol·L-1)模型组(Model)、和营安心方(500、250、125 mg·kg-1)三个剂量组,四甲基偶氮唑盐(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide,MTT)代谢率检测心肌细胞细胞活性,测定细胞培养液中乳酸脱氢酶(lactate dehydrogenase,LDH)及丙二醛(malondialdehyde,MDA)的含量。结果:H2O2组细胞活性显著性降低(P<0.01),LDH及MDA含量显著性升高(P<0.01);和营安心方可显著提高H2O2损伤心肌细胞的活性,减少MDA含量(P<0.05),降低LDH活性(P<0.05)。结论:和营安心方对H2O2损伤的心肌细胞具有明显的保护作用,其机制可能与抑制脂质过氧化有关。

Objective:To investigate the protective effects and mechanisms of He Ying An Xin Fang on myocardium injury induced by H2O2. Methods:Hydrogen peroxide(H2O2)was adopted to establish the rat embryonic cardiomyocytes oxidative stress insult model,and the experiment was divided into 5 groups:cardiomyocytes were incubated by H2O2(200μmol·L-1)for 2 h as model group,the non-H2O2 as control group,and the extraction of He Ying An Xin Fang(500,250,125 mg·kg-1)as therapeutic groups. The cell viability by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide(MTT), content of malondialdehyde(MDA)and activity of lactate dehydrogenase(LDH)were measured. Resulds:Cell viability by MTT:compared with control group,cell viability by MTT was decreased significantly in H2O2 group(P<0.01),while He Ying An Xin Fang had no significant effect on cardiac myocytes of cell viability. Content of MDA:compared with that of H2O2 group,the content of MDA was decreased in He Ying An Xin Fang groups significantly(P<0.05). Content of LDH:compared with that of control group,the content of LDH was decreased in He Ying An Xin Fang groups significantly (P<0.05). Conclusion:He Ying An Xin Fang has significant protective effects on free radical injury induced by H2O2 in myocardium rats,and it may be related to the inhibition of lipid peroxidation.