环境样品中军团菌定量检测方法

Quantitative Detection of Legionella in Environmental Samples

目的 研究从环境样品水和土壤中分离培养军团菌的定量检测方法。方法 应用阴性样品加标回收实验探讨分离培养条件,提出方法的回收率、最低检出限与计算模式。结果 加标实验显示:水样品最低检出限为1 cfu/mL,当加标菌量为10 cfu/mL时,全部样品均可检出加标菌,回收率为81%~95%;土壤样品选用0.01%Tween 80-PBS作为洗脱液,最低检出限为100 cfu/g,当加标菌量为1 000 cfu/g时,全部样品均可检出加标菌,回收率30%~90%。结论 本研究提出了用于水和土壤等环境样品中的军团菌定量检测方法。

Objectives To develop a quantitative detection method for the isolation and culture of legionella in water and soil. Methods Using a recovery of standard addition test to explore a detected conditions for quantitative isolation and culture,recovery rate,limit of detection and calculation mode. Results The limits of detection for water and soil samples were 1 cfu /mL and 102cfu /g,the recoveries were in the range of 81%- 95%and 30%- 90%,respectively. Conclusions A quantitative detection method applied to isolate and culture legionella in water and soil was proposed in this study.